Digital Autofocus Methods for Automated Microscopy

Feimo Shen, Louis Hodgson, Klaus Hahn

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations


Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.

Original languageEnglish (US)
Title of host publicationMeasuring Biological Responses with Automated Microscopy
PublisherAcademic Press Inc.
Number of pages13
ISBN (Print)0121828190, 9780121828196
StatePublished - 2006
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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