Dietary 2-deoxy-D-glucose impairs tumour growth and metastasis by inhibiting angiogenesis

Saurabh Singh, Sanjay Pandey, Amanpreet Singh Chawla, Anant Narayan Bhatt, Bal Gangadhar Roy, Daman Saluja, Bilikere S. Dwarakanath

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Accumulating evidence suggests the antiangiogenic potential of the glycolytic inhibitor 2-deoxy-D-glucose (2-DG) among the anticancerous properties of this drug. In the present studies, we investigated the antiangiogenic effects of dietary 2-DG on tumour (Lewis lung carcinoma [LLC]) as well as ionising radiation–induced angiogenesis in mouse models. Dietary 2-DG reduced the serum vascular endothelial growth factor levels (∼40%) in LLC-bearing mice along with a significant inhibition of tumour growth and metastases. In vivo Matrigel plug assays showed significant decrease in vascularisation, Fluorescein isothiocyanate (FITC)-dextran fluorescence and factor VIII–positive cells in the plugs from 2-DG–fed mice, supporting the notion that dietary 2-DG significantly suppresses the tumour-associated and radiation-induced angiogenesis. 2-DG inhibited the glucose usage and lactate production as well as ATP levels of human umbilical vein endothelial cells (HUVECs) in a concentration-dependent manner, accompanied by growth inhibition and loss of viability in vitro. Furthermore, 2-DG inhibited the capillary-like tube formation in Matrigel as well as migration and transwell invasion by HUVECs, which are functional indicators of the process of angiogenesis. These results suggest that dietary 2-DG inhibits processes related to angiogenesis, which can impair the growth and metastasis of tumours.

Original languageEnglish (US)
Pages (from-to)11-24
Number of pages14
JournalEuropean Journal of Cancer
StatePublished - Dec 2019


  • 2-Deoxy-D-glucose
  • Energy restriction
  • Lewis lung carcinoma
  • Radiation-induced angiogenesis
  • Tumour-induced angiogenesis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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