TY - JOUR
T1 - Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins
AU - Yang, Zhihua
AU - Shcherbakova, Daria M.
AU - Verkhusha, Vladislav V.
AU - Houston, Jessica P.
N1 - Publisher Copyright:
© OSA 2016.
PY - 2016
Y1 - 2016
N2 - Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.
AB - Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.
UR - http://www.scopus.com/inward/record.url?scp=85136565311&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136565311&partnerID=8YFLogxK
M3 - Conference article
AN - SCOPUS:85136565311
SN - 2162-2701
JO - Optics InfoBase Conference Papers
JF - Optics InfoBase Conference Papers
M1 - SW4G.1
T2 - CLEO: Science and Innovations, SI 2016
Y2 - 5 June 2016 through 10 June 2016
ER -