TY - GEN
T1 - Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins
AU - Yang, Zhihua
AU - Shcherbakova, Daria M.
AU - Verkhusha, Vladislav V.
AU - Houston, Jessica P.
N1 - Funding Information:
This work was supported in part by grants 2U54CA132383-06A1, GM073913 and GM108579 from the National Institutes of Health
Publisher Copyright:
© 2016 OSA.
PY - 2016/12/16
Y1 - 2016/12/16
N2 - Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.
AB - Excited state fluorescence lifetimes of near-infrared fluorescent proteins iRFP682 and iRFP713 engineered from bacterial phytochromes were measured with a time-resolved flow cytometer. Average lifetimes ranged between 0.5 and 1.0 ns for n=5000 bacterial cells. We anticipate cell sorting for screening cells expressing mutated iRFP variants of high quantum yield.
UR - http://www.scopus.com/inward/record.url?scp=85010666925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85010666925&partnerID=8YFLogxK
U2 - 10.1364/cleo_si.2016.sw4g.1
DO - 10.1364/cleo_si.2016.sw4g.1
M3 - Conference contribution
AN - SCOPUS:85010666925
T3 - 2016 Conference on Lasers and Electro-Optics, CLEO 2016
BT - 2016 Conference on Lasers and Electro-Optics, CLEO 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 Conference on Lasers and Electro-Optics, CLEO 2016
Y2 - 5 June 2016 through 10 June 2016
ER -