Determination of binding effects of FN peptides with platelet derived growth factor (PDGF)

M. S. Fourman, F. Lin, R. A. Clark

Research output: Chapter in Book/Report/Conference proceedingConference contribution


PDGF is a crucial growth factor in the human body, playing a part in cellular activity such as cell proliferation/migration, and angiogenesis. It is also linked to diseases such as atherosclerosis. Here we apply PDGF-BB to fibronectin domain binding. Four Peptides were tested originating from the first FN type III repeat (FNIII1), the heparinII-binding domain (FNIII12-15) and the variably spliced IIICS domain. Circular dichroism was used to determine binding effects of PDGF to these peptides, evaluating the difference in signal between the addition of the independent peptides and the combination of the two. Results indicate distinct signal change in several of the target peptides when added to PDGF, indicating binding. Such results can also indicate as to positive beta-sheet secondary structure formation. This formation will be analyzed using Dichroweb software from the Centre for Protein and Membrane Structure and Dynamics (CPMSD) in Daresbury Laboratory. Results will define binding trends of peptides to PDGF-BB, as well as clarify where binding on aforementioned domains best occurs.

Original languageEnglish (US)
Title of host publication33rd Annual Northeast Bioengineering Conference - Engineering Innovations in Life Sciences and Healthcare, NEBC
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages2
ISBN (Print)1424410339, 9781424410330
StatePublished - 2007
Externally publishedYes
Event33rd Annual Northeast Bioengineering Conference, NEBC - Stony Brook, NY, United States
Duration: Mar 10 2007Mar 11 2007

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X


Other33rd Annual Northeast Bioengineering Conference, NEBC
Country/TerritoryUnited States
CityStony Brook, NY

ASJC Scopus subject areas

  • Chemical Engineering(all)


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