Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator

Mikhail E. Matlashov, Jorge Vera, Ludmila A. Kasatkina, Kamran Khodakhah, Vladislav V. Verkhusha

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.

Original languageEnglish (US)
Article number880107
JournalFrontiers in Cell and Developmental Biology
StatePublished - Jun 29 2022


  • FRET
  • GECI
  • biosensor
  • calcium imaging
  • far-red
  • iRFP

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology


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