Abstract
Objective: To study protein-protein interaction between heterogeneous nuclear ribonucleoprotein H (hnRNP H) and Dengue virus (DENV) proteins. Methods: DENV proteins were screened against the host hnRNP H protein, in order to identify the host-viral protein-protein interactions in DENV infected THP-1 cells by co-immunoprecipitation. The co-localization of the interacting proteins was further confirmed by immunofluorescence microscopy. Results: The host protein hnRNP H was found to interact with DENV non-structural 1 protein and help the virus to multiply in the cell. Conclusions: The non-structural 1 glycoprotein is a key modulator of host immune response and is also involved in viral replication. Therefore, disruption of this key interaction between hnRNP H and DENV non-structural 1 could be an important therapeutic strategy for management of DENV infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 112-118 |
| Number of pages | 7 |
| Journal | Asian Pacific Journal of Tropical Medicine |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2016 |
| Externally published | Yes |
Keywords
- Dengue virus
- Heterogeneous nuclear ribonucleoprotein H
- Non-structural 1 protein
- Protein interactions
ASJC Scopus subject areas
- General Medicine
