Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus

M. Grazziutti; D. Przepiorka; J. H. Rex; I. Braunschweig; S. Vadhan-Raj; C. A. Savary

doi:10.1038/sj.bmt.1702832

Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus

M. Grazziutti, D. Przepiorka, J. H. Rex, I. Braunschweig, S. Vadhan-Raj, C. A. Savary

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Lymphocytes play a major role in host defense against Aspergillus, but little is known about the contribution of dendritic cells (DC) to antifungal immunity in humans. We have observed that DC derived from normal volunteers phagocytose heat-killed A. fumigatus conidia. Following 24 h of exposure to the fungus, DC displayed an increase in the mean fluorescence intensity of HLA-DR, CD80, and CD86, and an increase in the percentage of CD54⁺ cells. These DC also displayed increased production of IL-12. DC derived from CD34⁺ progenitors or monocytes stimulated autologous lymphocytes to proliferate and produce high levels of interferon-γ, but not interleukin-10, in response to fungal antigen. DC generated from CD34⁺ progenitors collected prior to autologous or allogeneic stem cell transplantation also partially restored the in vitro antifungal proliferative response of lymphocytes obtained from patients 1 month after transplantation. These results suggest that DC are important to host-response to A. fumigatus, and that ex vivo-generated DC might be useful in restoring or enhancing the antifungal immunity after hematopoietic stem cell transplantation.

Original language	English (US)
Pages (from-to)	647-652
Number of pages	6
Journal	Bone Marrow Transplantation
Volume	27
Issue number	6
DOIs	https://doi.org/10.1038/sj.bmt.1702832
State	Published - 2001
Externally published	Yes

Keywords

Aspergillus
Candida
Dendritic cell
Fungi
Immune reconstitution

ASJC Scopus subject areas

Hematology
Transplantation

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10.1038/sj.bmt.1702832

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title = "Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus",

abstract = "Lymphocytes play a major role in host defense against Aspergillus, but little is known about the contribution of dendritic cells (DC) to antifungal immunity in humans. We have observed that DC derived from normal volunteers phagocytose heat-killed A. fumigatus conidia. Following 24 h of exposure to the fungus, DC displayed an increase in the mean fluorescence intensity of HLA-DR, CD80, and CD86, and an increase in the percentage of CD54+ cells. These DC also displayed increased production of IL-12. DC derived from CD34+ progenitors or monocytes stimulated autologous lymphocytes to proliferate and produce high levels of interferon-γ, but not interleukin-10, in response to fungal antigen. DC generated from CD34+ progenitors collected prior to autologous or allogeneic stem cell transplantation also partially restored the in vitro antifungal proliferative response of lymphocytes obtained from patients 1 month after transplantation. These results suggest that DC are important to host-response to A. fumigatus, and that ex vivo-generated DC might be useful in restoring or enhancing the antifungal immunity after hematopoietic stem cell transplantation.",

keywords = "Aspergillus, Candida, Dendritic cell, Fungi, Immune reconstitution",

author = "M. Grazziutti and D. Przepiorka and Rex, {J. H.} and I. Braunschweig and S. Vadhan-Raj and Savary, {C. A.}",

note = "Funding Information: This work was supported in part by grant NAG8–1347 from the National Aeronautics and Space Administration (NASA), subcontract NCC 9–36 under the Texas Medical Center – NASA/Johnson Space Center Cooperative Agreement, grant CA-16672 from the National Cancer Institute, and the Tony Anderson Fund.",

year = "2001",

doi = "10.1038/sj.bmt.1702832",

language = "English (US)",

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T1 - Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus

AU - Grazziutti, M.

AU - Przepiorka, D.

AU - Rex, J. H.

AU - Braunschweig, I.

AU - Vadhan-Raj, S.

AU - Savary, C. A.

N1 - Funding Information: This work was supported in part by grant NAG8–1347 from the National Aeronautics and Space Administration (NASA), subcontract NCC 9–36 under the Texas Medical Center – NASA/Johnson Space Center Cooperative Agreement, grant CA-16672 from the National Cancer Institute, and the Tony Anderson Fund.

PY - 2001

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N2 - Lymphocytes play a major role in host defense against Aspergillus, but little is known about the contribution of dendritic cells (DC) to antifungal immunity in humans. We have observed that DC derived from normal volunteers phagocytose heat-killed A. fumigatus conidia. Following 24 h of exposure to the fungus, DC displayed an increase in the mean fluorescence intensity of HLA-DR, CD80, and CD86, and an increase in the percentage of CD54+ cells. These DC also displayed increased production of IL-12. DC derived from CD34+ progenitors or monocytes stimulated autologous lymphocytes to proliferate and produce high levels of interferon-γ, but not interleukin-10, in response to fungal antigen. DC generated from CD34+ progenitors collected prior to autologous or allogeneic stem cell transplantation also partially restored the in vitro antifungal proliferative response of lymphocytes obtained from patients 1 month after transplantation. These results suggest that DC are important to host-response to A. fumigatus, and that ex vivo-generated DC might be useful in restoring or enhancing the antifungal immunity after hematopoietic stem cell transplantation.

AB - Lymphocytes play a major role in host defense against Aspergillus, but little is known about the contribution of dendritic cells (DC) to antifungal immunity in humans. We have observed that DC derived from normal volunteers phagocytose heat-killed A. fumigatus conidia. Following 24 h of exposure to the fungus, DC displayed an increase in the mean fluorescence intensity of HLA-DR, CD80, and CD86, and an increase in the percentage of CD54+ cells. These DC also displayed increased production of IL-12. DC derived from CD34+ progenitors or monocytes stimulated autologous lymphocytes to proliferate and produce high levels of interferon-γ, but not interleukin-10, in response to fungal antigen. DC generated from CD34+ progenitors collected prior to autologous or allogeneic stem cell transplantation also partially restored the in vitro antifungal proliferative response of lymphocytes obtained from patients 1 month after transplantation. These results suggest that DC are important to host-response to A. fumigatus, and that ex vivo-generated DC might be useful in restoring or enhancing the antifungal immunity after hematopoietic stem cell transplantation.

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KW - Candida

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KW - Immune reconstitution

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Dendritic cell-mediated stimulation of the in vitro lymphocyte response to Aspergillus

Abstract

Keywords

ASJC Scopus subject areas

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