@article{cd769aafdf5b4c208d19d796a734d77f,
title = "Definition of a small core transcriptional circuit regulated by AML1-ETO",
abstract = "Stengel et al. combine PROTAC-mediated degradation of the oncogenic transcription factor AML1-ETO with nascent transcript analysis to identify direct gene targets. AML1-ETO-mediated repression of this small gene network impairs myeloid differentiation. This provides a model for the identification of transcriptional circuits directly controlled by transcription factors.",
keywords = "AML1-ETO, PRO-seq, PROTAC, RUNX1, RUNX1T1, degron tag, myeloid leukemia, nascent transcription",
author = "Stengel, {Kristy R.} and Ellis, {Jacob D.} and Spielman, {Clare L.} and Bomber, {Monica L.} and Hiebert, {Scott W.}",
note = "Funding Information: We thank the members of the Hiebert lab for helpful discussions, reagents, and advice. We thank the VICC Flow Cytometry, Chemical Synthesis, and Genome Sciences Shared Resources for services and support. We also thank Mark Wunderlich and James Mulloy (Cincinnati Children{\textquoteright}s Hospital Medical Center) for helpful advice with the CD34 + cultures and Dr. H. Leighton Grimes (Cincinnati Children{\textquoteright}s Hospital Medical Center) for the Gfi1b plasmid. This work was supported by the T.J. Martell Foundation , the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation , the National Institutes of Health (grants RO1-CA178030 , RO1-CA164605 , and R01-CA64140 ), core services performed through Vanderbilt Digestive Disease Research grant NIDDK P30DK58404 and Vanderbilt-Ingram Cancer Center support grant NCI P30CA68485 ), and the National Center for Advancing Translational Sciences (grant 2 UL1 TR000445-06 ). K.R.S. was supported by American Cancer Society grant 5 T32 CA009582-26 and postdoctoral fellowship PF-13-303-01-DMC . Funding Information: We thank the members of the Hiebert lab for helpful discussions, reagents, and advice. We thank the VICC Flow Cytometry, Chemical Synthesis, and Genome Sciences Shared Resources for services and support. We also thank Mark Wunderlich and James Mulloy (Cincinnati Children's Hospital Medical Center) for helpful advice with the CD34+ cultures and Dr. H. Leighton Grimes (Cincinnati Children's Hospital Medical Center) for the Gfi1b plasmid. This work was supported by the T.J. Martell Foundation, the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation, the National Institutes of Health (grants RO1-CA178030, RO1-CA164605, and R01-CA64140), core services performed through Vanderbilt Digestive Disease Research grant NIDDK P30DK58404 and Vanderbilt-Ingram Cancer Center support grant NCI P30CA68485), and the National Center for Advancing Translational Sciences (grant 2 UL1 TR000445-06). K.R.S. was supported by American Cancer Society grant 5 T32 CA009582-26 and postdoctoral fellowship PF-13-303-01-DMC. Conceptualization, K.R.S. and S.W.H.; Methodology, K.R.S. and S.W.H.; Investigation, K.R.S. J.D.E. C.L.S. M.L.B. and S.W.H.; Formal Analysis, M.L.B. C.L.S. and K.R.S.; Writing – Original Draft, K.R.S. and S.W.H.; Writing – Review & Editing, K.R.S. J.D.E. C.L.S. M.L.B. and S.W.H.; Visualization, K.R.S. and S.W.H.; Supervision, K.R.S. and S.W.H.; Funding Acquisition, S.W.H. The authors declare no competing interests, although S.W.H. received research funding from Incyte through the Vanderbilt-Incyte Alliance. These funds did not support this work. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = feb,
day = "4",
doi = "10.1016/j.molcel.2020.12.005",
language = "English (US)",
volume = "81",
pages = "530--545.e5",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}