Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease

Michael E.T.I. Boerrigter; Cornelia M. Van Duijn; Erik Mullaart; Piet Eikelenboom; Cees M.A. Van Der Togt; Dick L. Knook; Albert Hofman; Jan Vijg

doi:10.1016/0197-4580(91)90024-E

Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease

Michael E.T.I. Boerrigter, Cornelia M. Van Duijn, Erik Mullaart, Piet Eikelenboom, Cees M.A. Van Der Togt, Dick L. Knook, Albert Hofman, Jan Vijg

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 ± 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 ± 8.2% and 43.0 ± 4.4%, respectively; p>0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 ± 5.8%, which was significantly lower than that found in controls (p<0.01) or in the other AD patients (p<0.02).

Original language	English (US)
Pages (from-to)	367-370
Number of pages	4
Journal	Neurobiology of Aging
Volume	12
Issue number	4
DOIs	https://doi.org/10.1016/0197-4580(91)90024-E
State	Published - 1991
Externally published	Yes

Keywords

Alkaline elution
Alzheimer
DNA repair
Ethylnitrosourea
Lymphocytes

ASJC Scopus subject areas

Neuroscience(all)
Aging
Developmental Biology
Clinical Neurology
Geriatrics and Gerontology

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10.1016/0197-4580(91)90024-E

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title = "Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease",

abstract = "Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 ± 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 ± 8.2% and 43.0 ± 4.4%, respectively; p>0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 ± 5.8%, which was significantly lower than that found in controls (p<0.01) or in the other AD patients (p<0.02).",

keywords = "Alkaline elution, Alzheimer, DNA repair, Ethylnitrosourea, Lymphocytes",

author = "Boerrigter, {Michael E.T.I.} and {Van Duijn}, {Cornelia M.} and Erik Mullaart and Piet Eikelenboom and {Van Der Togt}, {Cees M.A.} and Knook, {Dick L.} and Albert Hofman and Jan Vijg",

note = "Funding Information: The major part of this work was supported by grants from Senetek p.l.c, and the Dutch Ministry of Welfare, Health and Cultural Affairs. Additional support was obtained from the Sandoz Foundation for Gerontological Research, the SOOM Foundation, the Netherlands Organisation for Pure Research and Sigma-Tau.",

year = "1991",

doi = "10.1016/0197-4580(91)90024-E",

language = "English (US)",

volume = "12",

pages = "367--370",

journal = "Neurobiology of Aging",

issn = "0197-4580",

publisher = "Elsevier Inc.",

number = "4",

}

TY - JOUR

T1 - Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease

AU - Boerrigter, Michael E.T.I.

AU - Van Duijn, Cornelia M.

AU - Mullaart, Erik

AU - Eikelenboom, Piet

AU - Van Der Togt, Cees M.A.

AU - Knook, Dick L.

AU - Hofman, Albert

AU - Vijg, Jan

N1 - Funding Information: The major part of this work was supported by grants from Senetek p.l.c, and the Dutch Ministry of Welfare, Health and Cultural Affairs. Additional support was obtained from the Sandoz Foundation for Gerontological Research, the SOOM Foundation, the Netherlands Organisation for Pure Research and Sigma-Tau.

PY - 1991

Y1 - 1991

N2 - Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 ± 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 ± 8.2% and 43.0 ± 4.4%, respectively; p>0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 ± 5.8%, which was significantly lower than that found in controls (p<0.01) or in the other AD patients (p<0.02).

AB - Using the alkaline filter elution technique we determined the induction and disappearance of DNA single-strand breaks (SSB) in freshly isolated peripheral blood lymphocytes (PBL) from 43 Alzheimer's disease (AD) patients and 48 normal, healthy age- and sex-matched control subjects following in vitro exposure to N-ethyl-N-nitrosourea (ENU). The mean percentage SSB disappearance in PBL from control subjects at 1 h after ENU treatment was 41.4 ± 2.9%; this was not significantly different from that found in samples from AD patients which had no (n = 16) or one (n = 12) first-degree relative with dementia (42.5 ± 8.2% and 43.0 ± 4.4%, respectively; p>0.75). However, in PBL of 15 AD patients with at least two first-degree relatives with dementia the mean percentage SSB disappearance was 23.6 ± 5.8%, which was significantly lower than that found in controls (p<0.01) or in the other AD patients (p<0.02).

KW - Alkaline elution

KW - Alzheimer

KW - DNA repair

KW - Ethylnitrosourea

KW - Lymphocytes

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JO - Neurobiology of Aging

JF - Neurobiology of Aging

IS - 4

ER -

Decreased DNA repair capacity in familial, but not in sporadic Alzheimer's disease

Abstract

Keywords

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