Cytokine regulation of iNOS expression in human glial cells

Sunhee C. Lee, Celia F. Brosnan

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


In this report, we review the evidence for the production of nitric oxide following cytokine activation of the inducible form of nitric oxide synthase by central nervous system glial cells, with particular reference to cells of human origin. The results suggest that cytokine regulation of this enzyme in human glial cells differs significantly from that found in cells of rodent or murine origin, with astrocytes rather than microglia being the major source of these products in inflamed nervous system tissue. The key activator of this enzyme in human astrocytes is IL-1 with IFN-γ acting synergistically. In contrast to glial cells of rodent or murine origin, no down-regulatory effects were noted with the cytokines IL-4, IL-10, and TGF-β. The relevance of these data to human neuropathology is discussed.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalMethods: A Companion to Methods in Enzymology
Issue number1
StatePublished - Aug 1996

ASJC Scopus subject areas

  • Molecular Biology
  • General Biochemistry, Genetics and Molecular Biology


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