Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus

Matthew W. Vetting; Cathleen A. Earhart; Douglas H. Ohlendorf

doi:10.1006/jmbi.1994.1142

Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus

Matthew W. Vetting, Cathleen A. Earhart, Douglas H. Ohlendorf

Biochemistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe⁺³)₁₂ complex.

Original language	English (US)
Pages (from-to)	372-373
Number of pages	2
Journal	Journal of Molecular Biology
Volume	236
Issue number	1
DOIs	https://doi.org/10.1006/jmbi.1994.1142
State	Published - Feb 10 1994

Keywords

Acinetobacter calcoaceticus
Crystallization
Metalloenzymes
Protocatechuate 3,4-dioxygenase
X-ray diffraction

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1006/jmbi.1994.1142

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title = "Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus",

abstract = "X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 {\AA}. The dodecahedral crystals diffract to beyond 2.5 {\AA} resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex.",

keywords = "Acinetobacter calcoaceticus, Crystallization, Metalloenzymes, Protocatechuate 3,4-dioxygenase, X-ray diffraction",

author = "Vetting, {Matthew W.} and Earhart, {Cathleen A.} and Ohlendorf, {Douglas H.}",

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AU - Earhart, Cathleen A.

AU - Ohlendorf, Douglas H.

PY - 1994/2/10

Y1 - 1994/2/10

N2 - X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex.

AB - X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex.

KW - Acinetobacter calcoaceticus

KW - Crystallization

KW - Metalloenzymes

KW - Protocatechuate 3,4-dioxygenase

KW - X-ray diffraction

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Crystallization and preliminary X-ray analysis of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus

Abstract

Keywords

ASJC Scopus subject areas

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