TY - JOUR
T1 - Contribution of LILRB1 polymorphism and HLA-DRB1-shared epitope to rheumatoid arthritis
AU - Delgado De La Poza, Juan Francisco
AU - Cantó, Elisabet
AU - Díaz-Torné, César
AU - Ferrer Villahoz, Beatriz
AU - Martínez Carretero, M. Angeles
AU - López, Marta
AU - Geli, Carmen
AU - Díaz, César
AU - Rodríguez-Sánchez, José Luis
AU - Vidal, Silvia
N1 - Funding Information:
E.C. is a participant in the Program “Contratos de apoyo a la Investigacion del Sistema Nacional de Salud”. S.V. was supported by “Fondo Investigaciones Sanitarias” and participant in the Program for Stabilization of Investigators of the “Direccio d’Estrategia i Coordinacio del Departament Salut de la Generalitat de Catalunya”.
PY - 2011
Y1 - 2011
N2 - Objectives: The LILRB1 gene has recently been associated with rheumatoid arthritis (RA) susceptibility in HLA-DRB1-shared epitope (SE) negative Japanese individuals. Since the contribution of the LILRB1 polymorphism to RA susceptibility may vary among ethnic populations, we examined this association in a group of Caucasian patients. The frequency of LILRB1 alleles was also determined in patients according to the presence of DRB1-SE. Methods: Samples from 103 RA patients and 107 healthy controls were randomly collected. Polymorphism of the LILRB1 gene was analyzed by sequencing with primers that amplified intron 3 and exon 4. Results: The frequencies of LILRB1 alleles in RA patients did not differ from those of controls. However, when patients and controls were grouped according to SE, the PE-01/01 genotype was less frequent in negative-SE patients than in controls. Whereas SE is associated with higher anti-CCP antibody levels, as expected, the production of anti-CCP antibodies was lower in negative-SE patients with PE-01/01 genotype. Moreover, radiographic damage in hand and feet of SE-negative PE-01/01 patients was less severe than in patients with other genotypes. Conclusions: The participation of this LILRB1 polymorphism in the RA pathogenesis of this Caucasian cohort differed fromthat reported in a Japanese sample. Our findings suggest that the LILRB1-PE-01/01 genotype could exert a protective role in RA susceptibility and disease severity in the absence of SE.
AB - Objectives: The LILRB1 gene has recently been associated with rheumatoid arthritis (RA) susceptibility in HLA-DRB1-shared epitope (SE) negative Japanese individuals. Since the contribution of the LILRB1 polymorphism to RA susceptibility may vary among ethnic populations, we examined this association in a group of Caucasian patients. The frequency of LILRB1 alleles was also determined in patients according to the presence of DRB1-SE. Methods: Samples from 103 RA patients and 107 healthy controls were randomly collected. Polymorphism of the LILRB1 gene was analyzed by sequencing with primers that amplified intron 3 and exon 4. Results: The frequencies of LILRB1 alleles in RA patients did not differ from those of controls. However, when patients and controls were grouped according to SE, the PE-01/01 genotype was less frequent in negative-SE patients than in controls. Whereas SE is associated with higher anti-CCP antibody levels, as expected, the production of anti-CCP antibodies was lower in negative-SE patients with PE-01/01 genotype. Moreover, radiographic damage in hand and feet of SE-negative PE-01/01 patients was less severe than in patients with other genotypes. Conclusions: The participation of this LILRB1 polymorphism in the RA pathogenesis of this Caucasian cohort differed fromthat reported in a Japanese sample. Our findings suggest that the LILRB1-PE-01/01 genotype could exert a protective role in RA susceptibility and disease severity in the absence of SE.
KW - Arthritis
KW - LILRB1
KW - Polymorphism
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U2 - 10.1016/j.inmuno.2011.06.004
DO - 10.1016/j.inmuno.2011.06.004
M3 - Article
AN - SCOPUS:84859009420
SN - 0213-9626
VL - 30
SP - 108
EP - 114
JO - Inmunologia
JF - Inmunologia
IS - 4
ER -