Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

Howard M. Steinman

doi:10.1007/BF00266247

Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

Howard M. Steinman

Biochemistry

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

Original language	English (US)
Pages (from-to)	427-430
Number of pages	4
Journal	MGG Molecular & General Genetics
Volume	232
Issue number	3
DOIs	https://doi.org/10.1007/BF00266247
State	Published - Apr 1992

Keywords

Deletion mutants
Escherichia coli
Legionella pneumophila
Superoxide dismutase

ASJC Scopus subject areas

Genetics

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10.1007/BF00266247

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abstract = "An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.",

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N2 - An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

AB - An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

KW - Deletion mutants

KW - Escherichia coli

KW - Legionella pneumophila

KW - Superoxide dismutase

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Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

Abstract

Keywords

ASJC Scopus subject areas

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