Abstract
Confocal laser scanning microscopy (CLSM) is a preferred method for obtaining optical images with submicrometer resolution. Replacing the pinhole and detector of a CLSM with a digital camera [charge-coupled device (CCD) or complementary metal oxide semiconductor (CMOS)] has the potential to simplify the design and reduce cost. However, the relatively slow speed of a typical camera results in long scans. To address this issue, in the present investigation a microlens array was used to split the laser beam into 48 beamlets that are focused onto the sample. In essence, 48 pinhole-detector measurements were performed in parallel. Images obtained from the 48 laser spots were stitched together into a final image.
Original language | English (US) |
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Pages (from-to) | 3058-3063 |
Number of pages | 6 |
Journal | Applied Optics |
Volume | 59 |
Issue number | 10 |
DOIs | |
State | Published - Apr 1 2020 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Engineering (miscellaneous)
- Electrical and Electronic Engineering