TY - JOUR
T1 - Comparison of BIOMED-2 versus laboratory-developed polymerase chain reaction assays for detecting T-cell receptor-γ gene rearrangements
AU - Patel, Keyur P.
AU - Pan, Qiulu
AU - Wang, Yanhua
AU - Maitta, Robert W.
AU - Du, Juan
AU - Xue, Xiaonan
AU - Lin, Juan
AU - Ratech, Howard
PY - 2010/3
Y1 - 2010/3
N2 - Detecting clonal T-cell receptor (TCR)-γ gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR-γ GR. The combination of TCR-β, mono-V TCR-γ and multi-V TCR-γ detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR-β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR-γ primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR-γ primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR-γ primers with TCR-β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR-γ V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.
AB - Detecting clonal T-cell receptor (TCR)-γ gene rearrangements (GRs) is an important adjunct test for diagnosing T-cell lymphoma. We compared a recently described assay (BIOMED-2 protocol), which targets multiple variable (V) gene segments in two polymerase chain reaction (PCR) reactions (multi-V), with a frequently referenced assay that targets a single V gene segment in four separate PCR reactions (mono-V). A total of 144 consecutive clinical DNA samples were prospectively tested for T-cell clonality by PCR using laboratory-developed mono-V and commercial multi-V primer sets for TCR-γ GR. The combination of TCR-β, mono-V TCR-γ and multi-V TCR-γ detected more clonal cases (68/144, 47%) than any individual PCR assay. We detected clonal TCR-β GR in 47/68 (69%) cases. Using either mono-V or multi-V TCR-γ primers, the sensitivities for detecting clonality were 52/68 (76%) or 51/68 (75%). Using both mono-V and multi-V TCR-γ primers improved the sensitivity for detecting clonality, 60/68 (88%). Combining either mono-V or multi-V TCR-γ primers with TCR-β primers also improved the sensitivity, 64/68 (94%). Significantly, TCR-γ V11 GRs could only be detected using the mono-V-PCR primers. We conclude that using more than one T-cell PCR assay can enhance the overall sensitivity for detecting T-cell clonality.
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U2 - 10.2353/jmoldx.2010.090042
DO - 10.2353/jmoldx.2010.090042
M3 - Article
C2 - 20181819
AN - SCOPUS:77649117978
SN - 1525-1578
VL - 12
SP - 226
EP - 237
JO - Journal of Molecular Diagnostics
JF - Journal of Molecular Diagnostics
IS - 2
ER -