Comparative population structure of Plasmodium falciparum circumsporozoite protein NANP repeat lengths in Lilongwe, Malawi

Natalie M. Bowman; Seth Congdon; Tisungane Mvalo; Jaymin C. Patel; Veronica Escamilla; Michael Emch; Francis Martinson; Irving Hoffman; Steven R. Meshnick; Jonathan J. Juliano

doi:10.1038/srep01990

Comparative population structure of Plasmodium falciparum circumsporozoite protein NANP repeat lengths in Lilongwe, Malawi

Natalie M. Bowman, Seth Congdon, Tisungane Mvalo, Jaymin C. Patel, Veronica Escamilla, Michael Emch, Francis Martinson, Irving Hoffman, Steven R. Meshnick, Jonathan J. Juliano

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Humoral immunity to Plasmodium falciparum circumsporozoite protein is partly mediated by a polymorphic NANP tetra-amino acid repeat. Antibody response to these repeats is the best correlate of protective immunity to the RTS,S malaria vaccine, but few descriptions of the natural variation of these repeats exist. Using capillary electrophoresis to determine the distribution of NANP repeat size polymorphisms among 98 isolates from Lilongwe, Malawi, we characterised the diversity of P. falciparum infection by several ecological indices. Infection by multiple distinct variants was common, and 20 distinct repeat sizes were identified. Diversity of P. falciparum appeared greater in children (18 variants) than adults (12 variants). There was evidence of genetic distance between different geographic regions by Nei's Standard Genetic Distance, suggesting parasite populations vary locally. We show that P. falciparum is very diverse with respect to NANP repeat length even on a local level and that diversity appears higher in children.

Original language	English (US)
Article number	1990
Journal	Scientific reports
Volume	3
DOIs	https://doi.org/10.1038/srep01990
State	Published - 2013
Externally published	Yes

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{7f1af000494c44f99f47c725f26c9e83,

title = "Comparative population structure of Plasmodium falciparum circumsporozoite protein NANP repeat lengths in Lilongwe, Malawi",

abstract = "Humoral immunity to Plasmodium falciparum circumsporozoite protein is partly mediated by a polymorphic NANP tetra-amino acid repeat. Antibody response to these repeats is the best correlate of protective immunity to the RTS,S malaria vaccine, but few descriptions of the natural variation of these repeats exist. Using capillary electrophoresis to determine the distribution of NANP repeat size polymorphisms among 98 isolates from Lilongwe, Malawi, we characterised the diversity of P. falciparum infection by several ecological indices. Infection by multiple distinct variants was common, and 20 distinct repeat sizes were identified. Diversity of P. falciparum appeared greater in children (18 variants) than adults (12 variants). There was evidence of genetic distance between different geographic regions by Nei's Standard Genetic Distance, suggesting parasite populations vary locally. We show that P. falciparum is very diverse with respect to NANP repeat length even on a local level and that diversity appears higher in children.",

author = "Bowman, {Natalie M.} and Seth Congdon and Tisungane Mvalo and Patel, {Jaymin C.} and Veronica Escamilla and Michael Emch and Francis Martinson and Irving Hoffman and Meshnick, {Steven R.} and Juliano, {Jonathan J.}",

note = "Funding Information: We would like to thank John Bruno and Feng Chang for their helpful suggestions and critical review of the analyses. We also thank the staff of the KCH pediatric and adult outpatient clinics, the staff of the UNC Project laboratory, and the study participants who were generous with their time and the hope for a malaria free Malawi. Natalie Bowman is supported by Ruth Kirschstein NRSA T32 715134. This work was supported by the National Institutes of Health grants 1RO1AI089819, KL2RR025746, and UL1RR025747.",

year = "2013",

doi = "10.1038/srep01990",

language = "English (US)",

volume = "3",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

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T1 - Comparative population structure of Plasmodium falciparum circumsporozoite protein NANP repeat lengths in Lilongwe, Malawi

AU - Bowman, Natalie M.

AU - Congdon, Seth

AU - Mvalo, Tisungane

AU - Patel, Jaymin C.

AU - Escamilla, Veronica

AU - Emch, Michael

AU - Martinson, Francis

AU - Hoffman, Irving

AU - Meshnick, Steven R.

AU - Juliano, Jonathan J.

N1 - Funding Information: We would like to thank John Bruno and Feng Chang for their helpful suggestions and critical review of the analyses. We also thank the staff of the KCH pediatric and adult outpatient clinics, the staff of the UNC Project laboratory, and the study participants who were generous with their time and the hope for a malaria free Malawi. Natalie Bowman is supported by Ruth Kirschstein NRSA T32 715134. This work was supported by the National Institutes of Health grants 1RO1AI089819, KL2RR025746, and UL1RR025747.

PY - 2013

Y1 - 2013

N2 - Humoral immunity to Plasmodium falciparum circumsporozoite protein is partly mediated by a polymorphic NANP tetra-amino acid repeat. Antibody response to these repeats is the best correlate of protective immunity to the RTS,S malaria vaccine, but few descriptions of the natural variation of these repeats exist. Using capillary electrophoresis to determine the distribution of NANP repeat size polymorphisms among 98 isolates from Lilongwe, Malawi, we characterised the diversity of P. falciparum infection by several ecological indices. Infection by multiple distinct variants was common, and 20 distinct repeat sizes were identified. Diversity of P. falciparum appeared greater in children (18 variants) than adults (12 variants). There was evidence of genetic distance between different geographic regions by Nei's Standard Genetic Distance, suggesting parasite populations vary locally. We show that P. falciparum is very diverse with respect to NANP repeat length even on a local level and that diversity appears higher in children.

AB - Humoral immunity to Plasmodium falciparum circumsporozoite protein is partly mediated by a polymorphic NANP tetra-amino acid repeat. Antibody response to these repeats is the best correlate of protective immunity to the RTS,S malaria vaccine, but few descriptions of the natural variation of these repeats exist. Using capillary electrophoresis to determine the distribution of NANP repeat size polymorphisms among 98 isolates from Lilongwe, Malawi, we characterised the diversity of P. falciparum infection by several ecological indices. Infection by multiple distinct variants was common, and 20 distinct repeat sizes were identified. Diversity of P. falciparum appeared greater in children (18 variants) than adults (12 variants). There was evidence of genetic distance between different geographic regions by Nei's Standard Genetic Distance, suggesting parasite populations vary locally. We show that P. falciparum is very diverse with respect to NANP repeat length even on a local level and that diversity appears higher in children.

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Comparative population structure of Plasmodium falciparum circumsporozoite protein NANP repeat lengths in Lilongwe, Malawi

Abstract

ASJC Scopus subject areas

UN SDGs

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