Chromogranin, an integral membrane protein

J. Settleman, J. Nolan, R. H. Angeletti

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Chromogranin is the major soluble protein of the adrenal medulla chromaffin granule and is secreted upon nervous stimulation. Using antisera to pure chromogranin in immunoblotting procedures, we show that chromogranin is the major integral membrane protein as well. Extraction of chromaffin granule membranes with low salt, high salt, chelating agents, or calcium-containing solutions does not remove the chromogranin from the membranes. The membrane form of chromogranin can be purified on a C-18 semipreparative column using high pressure liquid chromatography. Amino-terminal sequence data indicate that the membrane and soluble forms of chromogranin are identical or very similar.

Original languageEnglish (US)
Pages (from-to)1641-1644
Number of pages4
JournalJournal of Biological Chemistry
Issue number3
StatePublished - 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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