TY - JOUR
T1 - CHoMP
T2 - A chemoenzymatic histology method using clickable probes
AU - Rouhanifard, Sara H.
AU - López-Aguilar, Aimé
AU - Wu, Peng
N1 - Publisher Copyright:
©2014 Wiley-VCH Verlag GmbH & Co.
PY - 2014/11/17
Y1 - 2014/11/17
N2 - The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.
AB - The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.
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U2 - 10.1002/cbic.201402433
DO - 10.1002/cbic.201402433
M3 - Review article
AN - SCOPUS:84915737528
SN - 1439-4227
VL - 15
SP - 2667
EP - 2673
JO - ChemBioChem
JF - ChemBioChem
IS - 18
ER -