@article{0ee4c85254de4e55a4370780a8cee9fc,
title = "Chemoenzymatic synthesis of the sialyl Lewis X glycan and its derivatives",
abstract = "A combination of recombinant FKP and α-(1→3)-fucosyltransferase allows the facile synthesis of the sialyl Lewis X tetrasaccharide glycan and its derivatives in excellent yield. In this system, the universal fucosyl donor, guanidine 5′-diphosphate-β-l-fucose (GDP-fucose), or its analogues can be generated in situ by cofactor recycling using pyruvate kinase.",
keywords = "Chemoenzymatic synthesis, Fucose, Sialic acid, Transferases",
author = "{Soriano del Amo}, David and Wei Wang and Christen Besanceney and Tianqing Zheng and Yizheng He and Brian Gerwe and Seidel, {Ronald D.} and Peng Wu",
note = "Funding Information: This work was supported by the National Institutes of Health ( 4R00 GM080585–03 ) and startup funds from Albert Einstein College of Medicine . Yizheng He was supported by the Albert Einstein Summer Undergraduate Research Program. BL21 cells harboring fkp in pET-16b were provided by Professor Laurie E. Comstock. We thank Professor John S. Blanchard and Ms. Alison Sikora for assistance in kinetics measurements. ",
year = "2010",
month = jun,
day = "16",
doi = "10.1016/j.carres.2010.03.032",
language = "English (US)",
volume = "345",
pages = "1107--1113",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier BV",
number = "9",
}