Characterizing CD43 expression in haematogones using multicolour flow cytometric analysis

Lawrence Tsao; Adriana I. Colovai; Jie Gen Jiang; Govind Bhagat; Bachir Alobeid

doi:10.1111/j.1365-2141.2005.05374.x

Characterizing CD43 expression in haematogones using multicolour flow cytometric analysis

Lawrence Tsao, Adriana I. Colovai, Jie Gen Jiang, Govind Bhagat, Bachir Alobeid

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Haematogones are precursor B cells commonly detected in small numbers in the bone marrow. Morphologically, haematogones can mimic lymphoblasts and are best distinguished using multicolour flow cytometry with antibody combinations. Haematogones show characteristic and reproducible patterns of antigen expression representing the B-cell maturation sequence. CD43 expression, widely seen in haematopoietic elements, has not been well characterized in haematogones. We demonstrate that CD43 is consistently expressed in haematogones in a reproducible pattern similar to that of CD10 when combined with CD20. We propose that in combination with other markers, CD43 can be useful in the identification of haematogones.

Original language	English (US)
Pages (from-to)	820-823
Number of pages	4
Journal	British Journal of Haematology
Volume	128
Issue number	6
DOIs	https://doi.org/10.1111/j.1365-2141.2005.05374.x
State	Published - Mar 2005
Externally published	Yes

Keywords

B-lymphoblasts
CD43
Haematogones
Multicolour flow cytometry
Precursor B cells

ASJC Scopus subject areas

Hematology

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10.1111/j.1365-2141.2005.05374.x

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title = "Characterizing CD43 expression in haematogones using multicolour flow cytometric analysis",

abstract = "Haematogones are precursor B cells commonly detected in small numbers in the bone marrow. Morphologically, haematogones can mimic lymphoblasts and are best distinguished using multicolour flow cytometry with antibody combinations. Haematogones show characteristic and reproducible patterns of antigen expression representing the B-cell maturation sequence. CD43 expression, widely seen in haematopoietic elements, has not been well characterized in haematogones. We demonstrate that CD43 is consistently expressed in haematogones in a reproducible pattern similar to that of CD10 when combined with CD20. We propose that in combination with other markers, CD43 can be useful in the identification of haematogones.",

keywords = "B-lymphoblasts, CD43, Haematogones, Multicolour flow cytometry, Precursor B cells",

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year = "2005",

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T1 - Characterizing CD43 expression in haematogones using multicolour flow cytometric analysis

AU - Tsao, Lawrence

AU - Colovai, Adriana I.

AU - Jiang, Jie Gen

AU - Bhagat, Govind

AU - Alobeid, Bachir

PY - 2005/3

Y1 - 2005/3

N2 - Haematogones are precursor B cells commonly detected in small numbers in the bone marrow. Morphologically, haematogones can mimic lymphoblasts and are best distinguished using multicolour flow cytometry with antibody combinations. Haematogones show characteristic and reproducible patterns of antigen expression representing the B-cell maturation sequence. CD43 expression, widely seen in haematopoietic elements, has not been well characterized in haematogones. We demonstrate that CD43 is consistently expressed in haematogones in a reproducible pattern similar to that of CD10 when combined with CD20. We propose that in combination with other markers, CD43 can be useful in the identification of haematogones.

AB - Haematogones are precursor B cells commonly detected in small numbers in the bone marrow. Morphologically, haematogones can mimic lymphoblasts and are best distinguished using multicolour flow cytometry with antibody combinations. Haematogones show characteristic and reproducible patterns of antigen expression representing the B-cell maturation sequence. CD43 expression, widely seen in haematopoietic elements, has not been well characterized in haematogones. We demonstrate that CD43 is consistently expressed in haematogones in a reproducible pattern similar to that of CD10 when combined with CD20. We propose that in combination with other markers, CD43 can be useful in the identification of haematogones.

KW - B-lymphoblasts

KW - CD43

KW - Haematogones

KW - Multicolour flow cytometry

KW - Precursor B cells

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Characterizing CD43 expression in haematogones using multicolour flow cytometric analysis

Abstract

Keywords

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