TY - JOUR
T1 - Characterization of prophages containing “evolved” Dit/Tal modules in the genome of Lactobacillus casei BL23
AU - Dieterle, María Eugenia
AU - Fina Martin, Joaquina
AU - Durán, Rosario
AU - Nemirovsky, Sergio I.
AU - Sanchez Rivas, Carmen
AU - Bowman, Charles
AU - Russell, Daniel
AU - Hatfull, Graham F.
AU - Cambillau, Christian
AU - Piuri, Mariana
N1 - Funding Information:
This work was partially supported by UBACYT 2014-2017 GC 20020130100444BA to MP. M.E.D. is a doctoral fellow of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET, Argentina).
Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobacillus casei BL23. All of them have mosaic architectures with homologous sequences to Streptococcus, Lactococcus, Lactobacillus, and Listeria phages or strains. Using a combination of quantitative real-time PCR, genomics, and proteomics, we showed that PLE2 and PLE3 can be induced—but with different kinetics—in the presence of mitomycin C, although PLE1 remains as a prophage. A structural analysis of the distal tail (Dit) and tail associated lysin (Tal) baseplate proteins of these prophages and other L. casei/paracasei phages and prophages provides evidence that carbohydrate-binding modules (CBM) located within these “evolved” proteins may replace receptor binding proteins (RBPs) present in other well-studied LAB phages. The detailed study of prophage induction in this prototype strain in combination with characterization of the proteins involved in host recognition will facilitate the design of new strategies for avoiding phage propagation in the dairy industry.
AB - Lactic acid bacteria (LAB) have many applications in food and industrial fermentations. Prophage induction and generation of new virulent phages is a risk for the dairy industry. We identified three complete prophages (PLE1, PLE2, and PLE3) in the genome of the well-studied probiotic strain Lactobacillus casei BL23. All of them have mosaic architectures with homologous sequences to Streptococcus, Lactococcus, Lactobacillus, and Listeria phages or strains. Using a combination of quantitative real-time PCR, genomics, and proteomics, we showed that PLE2 and PLE3 can be induced—but with different kinetics—in the presence of mitomycin C, although PLE1 remains as a prophage. A structural analysis of the distal tail (Dit) and tail associated lysin (Tal) baseplate proteins of these prophages and other L. casei/paracasei phages and prophages provides evidence that carbohydrate-binding modules (CBM) located within these “evolved” proteins may replace receptor binding proteins (RBPs) present in other well-studied LAB phages. The detailed study of prophage induction in this prototype strain in combination with characterization of the proteins involved in host recognition will facilitate the design of new strategies for avoiding phage propagation in the dairy industry.
KW - Bacteriophage
KW - Baseplate
KW - Lactobacillus casei
KW - Prophage
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U2 - 10.1007/s00253-016-7727-x
DO - 10.1007/s00253-016-7727-x
M3 - Article
C2 - 27448399
AN - SCOPUS:84979287743
SN - 0175-7598
VL - 100
SP - 9201
EP - 9215
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 21
ER -