TY - JOUR
T1 - Characterization of a Diverse Set of Conditionally Reprogrammed Head and Neck Cancer Cell Cultures
AU - Ow, Thomas J.
AU - Mehta, Vikas
AU - Li, Daniel
AU - Thomas, Carlos
AU - Shrivastava, Nitisha
AU - Kawachi, Nicole
AU - Gersten, Adam J.
AU - Zhu, Jing
AU - Schiff, Bradley A.
AU - Smith, Richard V.
AU - Rosenblatt, Gregory
AU - Augustine, Stelby
AU - Prystowsky, Michael B.
AU - Yin, Shanye
AU - Gavathiotis, Evripidis
AU - Guha, Chandan
N1 - Publisher Copyright:
© 2024 The American Laryngological, Rhinological and Otological Society, Inc.
PY - 2024
Y1 - 2024
N2 - Objective: To establish and characterize a diverse library of head and neck squamous cell cancer (HNSCC) cultures using conditional reprogramming (CR). Methods: Patients enrolled on an IRB-approved protocol to generate tumor cell cultures using CR methods. Tumor and blood samples were collected and clinical information was recorded. Successful CR cultures were validated against banked reference tumors with short tandem repeat genotyping. Cell morphology was archived with photodocumentation. Clinical and demographic factors were evaluated for associations with successful establishment of CR culture. Human papilloma virus (HPV) genotyping, clonogenic survival, MTT assays, spheroid growth, and whole exome sequencing were carried out in selected cultures. Results: Forty four patients were enrolled, with 31 (70%) successful CR cultures, 32% derived from patients who identified as Black and 61% as Hispanic. All major head and neck disease sites were represented, including 15 (48%) oral cavity and 8 (26%) p16-positive oropharynx cancers. Hispanic ethnicity and first primary tumors (vs. second primary or recurrent tumors) were significantly associated with successful CR culture. HPV expression was conserved in CR cultures, including CR-024, which carried a novel HPV-69 serotype. CR cultures were used to test cisplatin responses using MTT assays. Previous work has also demonstrated these models can be used to assess response to radiation and can be engrafted in mouse models. Whole exome sequencing demonstrated that CR cultures preserved tumor mutation burden and driver mutations. Conclusion: CR culture is highly successful in propagating HNSCC cells. This study included a high proportion of patients from underrepresented minority groups. Level of Evidence: Not Applicable Laryngoscope, 2024.
AB - Objective: To establish and characterize a diverse library of head and neck squamous cell cancer (HNSCC) cultures using conditional reprogramming (CR). Methods: Patients enrolled on an IRB-approved protocol to generate tumor cell cultures using CR methods. Tumor and blood samples were collected and clinical information was recorded. Successful CR cultures were validated against banked reference tumors with short tandem repeat genotyping. Cell morphology was archived with photodocumentation. Clinical and demographic factors were evaluated for associations with successful establishment of CR culture. Human papilloma virus (HPV) genotyping, clonogenic survival, MTT assays, spheroid growth, and whole exome sequencing were carried out in selected cultures. Results: Forty four patients were enrolled, with 31 (70%) successful CR cultures, 32% derived from patients who identified as Black and 61% as Hispanic. All major head and neck disease sites were represented, including 15 (48%) oral cavity and 8 (26%) p16-positive oropharynx cancers. Hispanic ethnicity and first primary tumors (vs. second primary or recurrent tumors) were significantly associated with successful CR culture. HPV expression was conserved in CR cultures, including CR-024, which carried a novel HPV-69 serotype. CR cultures were used to test cisplatin responses using MTT assays. Previous work has also demonstrated these models can be used to assess response to radiation and can be engrafted in mouse models. Whole exome sequencing demonstrated that CR cultures preserved tumor mutation burden and driver mutations. Conclusion: CR culture is highly successful in propagating HNSCC cells. This study included a high proportion of patients from underrepresented minority groups. Level of Evidence: Not Applicable Laryngoscope, 2024.
KW - cancer of head and neck
KW - cell culture techniques
KW - diversity, equity, inclusion
KW - human papillomavirus
KW - translational medical research
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U2 - 10.1002/lary.31236
DO - 10.1002/lary.31236
M3 - Article
C2 - 38288866
AN - SCOPUS:85183936795
SN - 0023-852X
JO - Laryngoscope
JF - Laryngoscope
ER -