Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier

Vanessa A. Fitsanakis; Greg Piccola; Judy L. Aschner; Michael Aschner

doi:10.1016/j.neuro.2005.06.004

Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier

Vanessa A. Fitsanakis, Greg Piccola, Judy L. Aschner, Michael Aschner

Pediatrics

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18 Scopus citations

Abstract

Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The ⁵⁴Mn²⁺ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of ⁵⁴Mn ²⁺. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.

Original language	English (US)
Pages (from-to)	60-70
Number of pages	11
Journal	Neurotoxicology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1016/j.neuro.2005.06.004
State	Published - Jan 2006

Keywords

Astrocyte-conditioned medium
Energy-dependence
Iron-dependence
Proton-dependence
Sodium-dependence

ASJC Scopus subject areas

Neuroscience(all)
Toxicology

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10.1016/j.neuro.2005.06.004

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title = "Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier",

abstract = "Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The 54Mn2+ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of 54Mn 2+. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.",

keywords = "Astrocyte-conditioned medium, Energy-dependence, Iron-dependence, Proton-dependence, Sodium-dependence",

author = "Fitsanakis, {Vanessa A.} and Greg Piccola and Aschner, {Judy L.} and Michael Aschner",

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AU - Piccola, Greg

AU - Aschner, Judy L.

AU - Aschner, Michael

PY - 2006/1

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N2 - Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The 54Mn2+ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of 54Mn 2+. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.

AB - Manganese (Mn), an essential elemental nutrient, is known to be neurotoxic at high occupational levels. We examined the transport of Mn across a monolayer of rat brain endothelial cell (RBE4) to evaluate whether an electromotive permeability mechanism is responsible for Mn transport across the blood-brain barrier (BBB). The 54Mn2+ apparent permeability and flux showed significant temperature-, energy- and pH-dependence, as well as partial sodium-dependence. Additionally, iron (Fe)-rich and Fe-deficient media significantly increased the apparent permeability of 54Mn 2+. Finally, Mn flux and permeability decreased when RBE4 cells were grown in astrocyte-conditioned media (ACM), compared to standard alpha-media. These data reinforce observations that transport of Mn across the BBB occurs in part through active transport process.

KW - Astrocyte-conditioned medium

KW - Energy-dependence

KW - Iron-dependence

KW - Proton-dependence

KW - Sodium-dependence

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Characteristics of manganese (Mn) transport in rat brain endothelial (RBE4) cells, an in vitro model of the blood-brain barrier

Abstract

Keywords

ASJC Scopus subject areas

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