Changes in the guanine nucleotide-binding proteins, Gi and go, during differentiation of 3T3-L1 cells

Peter Gierschik; Bernice Morrow; Graeme Milligan; Charles Rubin; Allen Spiegel

doi:10.1016/0014-5793(86)81233-9

Changes in the guanine nucleotide-binding proteins, G_i and g_o, during differentiation of 3T3-L1 cells

Peter Gierschik, Bernice Morrow, Graeme Milligan, Charles Rubin, Allen Spiegel

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, G_i, and the novel G-protein, G_o, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39-40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain G_o, as well as G_i, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in G_i and G_o may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.

Original language	English (US)
Pages (from-to)	103-106
Number of pages	4
Journal	FEBS Letters
Volume	199
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)81233-9
State	Published - Apr 7 1986

Keywords

Adenylate cyclase
Pertussis toxin
Signal transduction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(86)81233-9

Cite this

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title = "Changes in the guanine nucleotide-binding proteins, Gi and go, during differentiation of 3T3-L1 cells",

abstract = "Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, Gi, and the novel G-protein, Go, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39-40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain Go, as well as Gi, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in Gi and Go may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.",

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T1 - Changes in the guanine nucleotide-binding proteins, Gi and go, during differentiation of 3T3-L1 cells

AU - Gierschik, Peter

AU - Morrow, Bernice

AU - Milligan, Graeme

AU - Rubin, Charles

AU - Spiegel, Allen

PY - 1986/4/7

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N2 - Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, Gi, and the novel G-protein, Go, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39-40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain Go, as well as Gi, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in Gi and Go may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.

AB - Differentiation of 3T3-L1 cells from fibroblasts to adipocytes is accompanied by increased adenylate cyclase response to lipolytic agents. We used pertussis toxin and specific antibodies to measure the inhibitory guanine nucleotide-binding protein, Gi, and the novel G-protein, Go, in membranes from 3T3-L1 cells. Pertussis toxin-dependent labeling of a 39-40 kDa protein showed an initial 30% rise, followed by an 80% fall during differentiation. Immunoblots showed that 3T3-L1 cells contain Go, as well as Gi, and that changes in the former parallel the changes in pertussis toxin labeling. Changes in Gi and Go may contribute to altered adenylate cyclase response during 3T3-L1 cell differentiation.

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KW - Pertussis toxin

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