Changes in the G0 state of WI-38 fibroblasts at different times after confluence

L. H. Augenlicht, R. Baserga

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143 Scopus citations


Some events in the prereplicative phase of WI-38 human diploid fibroblasts stimulated to proliferate are found to be a function of the length of time the cells have been quiescent. At 5 days after plating, when the cells first become confluent, the prereplicative phase upon stimulation by a nutritional change is relatively short, DNA synthesis begins at 8 h after stimulation, and there is no increase in chromatin template activity. At 9 days after plating the prereplicative phase of stimulated cells is lengthened to 14 h and there is an increase in chromatin template activity within 1 h of stimulation. Finally, in 18-day cells, the prereplicative phase is lengthened even further to 20 h, and there is a lag after stimulation before the increase in chromatin template activity. It is proposed that confluent WI-38 cells initially arrest in G 1, subsequently pass into G 0, and continue to go deeper into G 0 as they remain quiescent.

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalExperimental Cell Research
Issue number2
StatePublished - Dec 1974
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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