TY - JOUR
T1 - Cellular basis of urothelial squamous metaplasia
T2 - Roles of lineage heterogeneity and cell replacement
AU - Liang, Feng Xia
AU - Bosland, Maarten C.
AU - Huang, Hongying
AU - Romih, Rok
AU - Baptiste, Solange
AU - Deng, Fang Ming
AU - Wu, Xue Ru
AU - Shapiro, Ellen
AU - Sun, Tung Tien
PY - 2005/12/5
Y1 - 2005/12/5
N2 - Although the epithelial lining of much of the mammalian urinary tract is known simply as the urothelium, this epithelium can be divided into at least three lineages of renal pelvis/ureter, bladder/trigone, and proximal urethra based on their embryonic origin, uroplakin content, keratin expression pattern, in vitro growth potential, and propensity to keratinize during vitamin A deficiency. Moreover, these cells remain phenotypically distinct even after they have been serially passaged under identical culture conditions, thus ruling out local mesenchymal influence as the sole cause of their in vivo differences. During vitamin A deficiency, mouse urothelium form multiple keratinized foci in proximal urethra probably originating from scattered K14-positive basal cells, and the keratinized epithelium expands horizontally to replace the surrounding normal urothelium. These data suggest that the urothelium consists of multiple cell lineages, that trigone urothelium is closely related to the urothelium covering the rest of the bladder, and that lineage heterogeneity coupled with cell migration/replacement form the cellular basis for urothelial squamous metaplasia.
AB - Although the epithelial lining of much of the mammalian urinary tract is known simply as the urothelium, this epithelium can be divided into at least three lineages of renal pelvis/ureter, bladder/trigone, and proximal urethra based on their embryonic origin, uroplakin content, keratin expression pattern, in vitro growth potential, and propensity to keratinize during vitamin A deficiency. Moreover, these cells remain phenotypically distinct even after they have been serially passaged under identical culture conditions, thus ruling out local mesenchymal influence as the sole cause of their in vivo differences. During vitamin A deficiency, mouse urothelium form multiple keratinized foci in proximal urethra probably originating from scattered K14-positive basal cells, and the keratinized epithelium expands horizontally to replace the surrounding normal urothelium. These data suggest that the urothelium consists of multiple cell lineages, that trigone urothelium is closely related to the urothelium covering the rest of the bladder, and that lineage heterogeneity coupled with cell migration/replacement form the cellular basis for urothelial squamous metaplasia.
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U2 - 10.1083/jcb.200505035
DO - 10.1083/jcb.200505035
M3 - Article
C2 - 16330712
AN - SCOPUS:28544434424
SN - 0021-9525
VL - 171
SP - 835
EP - 844
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -