Cell Adhesion and Proliferation on Sulfonated and Non-Modified Chitosan Films

Enrique Martínez-Campos, Ana Civantos, Juan Alfonso Redondo, Rodrigo Guzmán, Mónica Pérez-Perrino, Alberto Gallardo, Viviana Ramos, Inmaculada Aranaz

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Three types of chitosan-based films have been prepared and evaluated: a non-modified chitosan film bearing cationizable aliphatic amines and two films made of N-sulfopropyl chitosan derivatives bearing both aliphatic amines and negative sulfonate groups at different ratios. Cell adhesion and proliferation on chitosan films of C2C12 pre-myoblastic cells and B16 cells as tumoral model have been tested. A differential cell behavior has been observed on chitosan films due to their different surface modification. B16 cells have shown lower vinculin expression when cultured on sulfonated chitosan films. This study shows how the interaction among cells and material surface can be modulated by physicochemical characteristics of the biomaterial surface, altering tumoral cell adhesion and proliferation processes.

Original languageEnglish (US)
Pages (from-to)974-982
Number of pages9
JournalAAPS PharmSciTech
Issue number4
StatePublished - May 1 2017
Externally publishedYes


  • focal adhesion
  • melanoma
  • N-sulfopropyl chitosan
  • proliferation

ASJC Scopus subject areas

  • Pharmaceutical Science


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