Capillary differentiation of endothelial cells on microgrooved surfaces

Hua Wang; Shengfu Chen; Buddy D. Ratner; E. Helene Sage; Shaoyi Jiang

doi:10.1021/jp075746z

Capillary differentiation of endothelial cells on microgrooved surfaces

Hua Wang, Shengfu Chen, Buddy D. Ratner, E. Helene Sage, Shaoyi Jiang

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We investigated the capillary differentiation of endothelial cells cultured on microgrooved polydimethylsiloxane (PDMS) substrates coated with fibronectin. Bovine aortic endothelial (BAE) cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) on PDMS substrates produced photolithographically. Phase contrast images of BAE cells were taken to assess the overall morphology of cells. Confocal microscopy was used to examine the existence of lumen inside cord-like and tube-like structures developed at different stages of cell culture. Herein we show for the first time that microgrooved PDMS substrates of appropriate dimensions coated with fibronectin are capable of inducing capillary differentiation of endothelial cells in the absence of angiogenic growth factors.

Original language	English (US)
Pages (from-to)	14602-14606
Number of pages	5
Journal	Journal of Physical Chemistry C
Volume	111
Issue number	40
DOIs	https://doi.org/10.1021/jp075746z
State	Published - Oct 11 2007
Externally published	Yes

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Energy(all)
Physical and Theoretical Chemistry
Surfaces, Coatings and Films

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AU - Wang, Hua

AU - Chen, Shengfu

AU - Ratner, Buddy D.

AU - Sage, E. Helene

AU - Jiang, Shaoyi

PY - 2007/10/11

Y1 - 2007/10/11

N2 - We investigated the capillary differentiation of endothelial cells cultured on microgrooved polydimethylsiloxane (PDMS) substrates coated with fibronectin. Bovine aortic endothelial (BAE) cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) on PDMS substrates produced photolithographically. Phase contrast images of BAE cells were taken to assess the overall morphology of cells. Confocal microscopy was used to examine the existence of lumen inside cord-like and tube-like structures developed at different stages of cell culture. Herein we show for the first time that microgrooved PDMS substrates of appropriate dimensions coated with fibronectin are capable of inducing capillary differentiation of endothelial cells in the absence of angiogenic growth factors.

AB - We investigated the capillary differentiation of endothelial cells cultured on microgrooved polydimethylsiloxane (PDMS) substrates coated with fibronectin. Bovine aortic endothelial (BAE) cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) on PDMS substrates produced photolithographically. Phase contrast images of BAE cells were taken to assess the overall morphology of cells. Confocal microscopy was used to examine the existence of lumen inside cord-like and tube-like structures developed at different stages of cell culture. Herein we show for the first time that microgrooved PDMS substrates of appropriate dimensions coated with fibronectin are capable of inducing capillary differentiation of endothelial cells in the absence of angiogenic growth factors.

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Capillary differentiation of endothelial cells on microgrooved surfaces

Abstract

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