@article{4d7fecf2d807477e8d327dd5cdc52bee,
title = "Candida glabrata produces a melanin-like pigment that protects against stress conditions encountered during parasitism",
abstract = "Aim: Melanin has been linked to pathogenesis in several fungi. They often produce melanin-like pigments in the presence of L-dihydroxyphenylalanine (L-DOPA), but this is poorly studied in Candida glabrata. Methods & materials: C. glabrata was grown in minimal medium with or without L-DOPA supplementation and submitted to a chemical treatment with denaturant and hot acid. Results: C. glabrata turned black when grown in the presence of L-DOPA, whereas cells grown without L-DOPA supplementation remained white. Biophysical properties demonstrated that the pigment was melanin. Melanized C. glabrata cells were effectively protected from azoles and amphotericin B, incubation at 42°C and macrophage killing. Conclusion: In the presence of L-DOPA, C. glabrata produces melanin, increases antifungal resistance and enhances host survival.",
keywords = "Candida glabrata, L-DOPA, melanin, survival, virulence",
author = "Rodrigo Almeida-Paes and Figueiredo-Carvalho, {Maria H.G.} and {Da Silva}, {Leandro B.R.} and Gerfen, {Gary J.} and {De S Ara{\'u}jo}, {G. R.} and Susana Frases and Zancop{\'e}-Oliveira, {Rosely M.} and Nosanchuk, {Joshua D.}",
note = "Funding Information: This study was financed in part by the Coordenac¸{\~a}o de Aperfeic¸oamento de Pessoal de N{\'i}vel Superior - Brasil (Capes) - Finance Code 001 and by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq 449184/2014–5). RM Zancop{\'e}-Oliveira was supported in part by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq 302796/2017–7) and Fundac¸{\~a}o Carlos Chagas Filho de Amparo {\`a} Pesquisa do Estado do Rio de Janeiro (FAPERJ E-26/202.527/2019). LBR da Silva was supported in partbyFundac¸{\~a}odeAmparo{\`a}PesquisadoEstadodeS{\~a}oPaulo(FAPESP2018/25171-6and2019/20622-2).RAlmeida-Paeswas supported in part by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq 305487/2015–9) and Coordenac¸{\~a}o de Aperfeic¸oamento de Pessoal de Nivel Superior (CAPES 88887.372494/2019–00). JD Nosanchuk was supported in part by NIH NIAID grant AI52733. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2021 Future Medicine Ltd.",
year = "2021",
month = may,
doi = "10.2217/fmb-2020-0228",
language = "English (US)",
volume = "16",
pages = "509--520",
journal = "Future Microbiology",
issn = "1746-0913",
publisher = "Future Medicine Ltd.",
number = "7",
}