Abstract
A diverse set of SUMO target proteins has been identified. Therefore, there is a growing interest in studying sumoylation and SUMO interactions in cells. When the sumoylation of a protein or a SUMO interaction is suspected, a standard co-immunoprecipitation analysis using anti-SUMO and anti-target protein antibody is usually performed as a first step. However, the identification of endogenous sumoylated proteins is challenging because of the activity of isopeptidases, and often only a small fraction of a target protein is sumoylated at a given time. Here, we briefly summarize several important steps to ensure a successful co-immunoprecipitation analysis to detect possible sumoylation.
Original language | English (US) |
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Pages (from-to) | 95-97 |
Number of pages | 3 |
Journal | Analytical Biochemistry |
Volume | 477 |
DOIs | |
State | Published - May 15 2015 |
Keywords
- Co-immunoprecipitation
- In vitro sumoylation
- Isopeptidases
- Mass spectrometry
- SUMO
- Sumoylation
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology