Brugia malayi abundant larval transcript 2 protein treatment attenuates experimentally-induced colitis in mice

Vishal Khatri, Nitin Amdare, Ravi Shankar Yadav, Aaditya Tarnekar, Kalyan Goswami, Maryada Venkata Rami Reddy

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Helminths are known to modulate host’s immunity by suppressing host protective pro-inflammatory responses. Such immunomodulatory effects have been experimentally shown to have therapeutic implications in immune mediated disorders. In the present study, we have explored a filarial protein i.e. Brugia malayi recombinant abundant larval transcript 2 (rBmALT2) for its therapeutic effect in dextran sodium sulfate (DSS) induced colitis in mouse model. The immunomodulatory activity of rBmALT-2 was initially confirmed by demonstrating that it suppressed the lipopolysaccharide (LPS) induced nitric oxide synthesis and down-regulated the expression of pro-inflammatory cytokines in vitro by peritoneal exudate cells of mice. Treatment with rBmALT2 reduced severity of colitis associated with significant reduction in weight loss, disease activity, colon damage, mucosal edema and histopathological score including myeloperoxidase activity in colon tissues. rBmALT2 was comparatively more effective in attenuation of colitis when used in the preventive mode than when used for curative purpose. The therapeutic effect of rBmALT2 was found to be associated with downregulation of IFN-γ, IL-6, IL-17 and upregulation of IL-10 cytokines. These results provide strong experimental evidence that BmALT2 could be a potential alternative therapeutic agent in colitis.

Original languageEnglish (US)
Pages (from-to)732-739
Number of pages8
JournalIndian Journal of Experimental Biology
Issue number11
StatePublished - Nov 2015
Externally publishedYes


  • Dextran sodium sulfate (DSS)
  • Helminths
  • Nematodes
  • rBmALT2
  • Ulcerative colitis

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology


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