@article{f8b6d35b79e24adc82098a9a2f675fd3,
title = "BMP and Wnt Specify Hematopoietic Fate by Activation of the Cdx-Hox Pathway",
abstract = "The formation of blood in the embryo is dependent on bone morphogenetic protein (BMP), but how BMP signaling intersects with other regulators of hematopoietic development is unclear. Using embryonic stem (ES) cells, we show that BMP4 first induces ventral-posterior (V-P) mesoderm and subsequently directs mesodermal cells toward blood fate by activating Wnt3a and upregulating Cdx and Hox genes. When BMP signaling is blocked during this latter phase, enforced expression of either Cdx1 or Cdx4 rescues hematopoietic development, thereby placing BMP4 signaling upstream of the Cdx-Hox pathway. Wnt signaling cooperates in BMP-induced hemogenesis, and the Wnt effector LEF1 mediates BMP4 activation of Cdx genes. Our data suggest that BMP signaling plays two distinct and sequential roles during blood formation, initially as an inducer of mesoderm, and later to specify blood via activation of Wnt signaling and the Cdx-Hox pathway.",
keywords = "DEVBIO, STEMCELL",
author = "Claudia Lengerke and Sabine Schmitt and Bowman, {Teresa V.} and Jang, {Il Ho} and Leila Maouche-Chretien and Shannon McKinney-Freeman and Davidson, {Alan J.} and Matthias Hammerschmidt and Fabian Rentzsch and Green, {Jeremy B.A.} and Zon, {Leonard I.} and Daley, {George Q.}",
note = "Funding Information: This study was supported by grants from the NIH and the NIH Director's Pioneer Award of the NIH Roadmap for Medical Research. G.Q.D. is a recipient of the Burroughs Wellcome Fund Clinical Scientist Award in Translational Research. C.L. was supported by a fellowship from the Dr. Mildred Scheel Foundation for Cancer Research, S.S. by a long-term fellowship from the Human Frontier Science Program Organization, and S.M.-F. by a grant from the American Cancer Society. C.L., S.S., T.V.B., A.J.D., J.B.A.G., L.I.Z., and G.Q.D. conceived and designed the experiments. C.L., S.S., T.V.B., I.H.J., and A.J.D. performed the experiments. S.M.-F. and I.H.J. generated the inducible Cdx1 ES cell line. M.H. and F.R. generated the tg( bmp2b ) zebrafish line. C.L. wrote the paper. S.S., T.V.B., L.M.-C., A.J.D., J.B.A.G., L.I.Z., and G.Q.D. edited the paper. ",
year = "2008",
month = jan,
day = "10",
doi = "10.1016/j.stem.2007.10.022",
language = "English (US)",
volume = "2",
pages = "72--82",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "1",
}