Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of ‘affinity directed’ β ; crosslinkers of HBA

B. N. Manjula; R. P. Roy; P. K. Smith; A. S. Acharya

doi:10.3109/10731199409117907

Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of ‘affinity directed’ β ; crosslinkers of HBA

B. N. Manjula, R. P. Roy, P. K. Smith, A. S. Acharya

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the/3-chains and inhibited the dissociation of HbA into α β dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong ‘steering’ influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

Original language	English (US)
Pages (from-to)	747-752
Number of pages	6
Journal	Artificial Cells, Blood Substitutes, and Biotechnology
Volume	22
Issue number	3
DOIs	https://doi.org/10.3109/10731199409117907
State	Published - Jan 1994
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biomedical Engineering

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title = "Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of {\textquoteleft}affinity directed{\textquoteright} β ; crosslinkers of HBA",

abstract = "The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the/3-chains and inhibited the dissociation of HbA into α β dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong {\textquoteleft}steering{\textquoteright} influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.",

author = "Manjula, {B. N.} and Roy, {R. P.} and Smith, {P. K.} and Acharya, {A. S.}",

year = "1994",

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T1 - Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids

T2 - A new class of ‘affinity directed’ β ; crosslinkers of HBA

AU - Manjula, B. N.

AU - Roy, R. P.

AU - Smith, P. K.

AU - Acharya, A. S.

PY - 1994/1

Y1 - 1994/1

N2 - The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the/3-chains and inhibited the dissociation of HbA into α β dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong ‘steering’ influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

AB - The potential of sulfosuccinimidyl ester of suberic acid as an intramolecular crosslinker of HbA, directed to the positive charge rich domain of the protein (DPG pocket) has been investigated. The suberate ester introduced crosslinks between the/3-chains and inhibited the dissociation of HbA into α β dimers. The facile crosslinking reaction seen with this suberate ester as compared to the absence of crosslinking with the diaspirin derivative of suberic acid suggests the strong ‘steering’ influence of the sulfosuccinimidyl moiety of the reagent to the targeted site. The application of this crosslinking approach in the preparation of Hb based blood substitutes is discussed.

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Bissulfosuccinimidyl esters of aliphatic dicarboxylic acids: A new class of ‘affinity directed’ β ; crosslinkers of HBA

Abstract

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