A systems mechanical analysis of biological samples, particularly DNA molecules and synthetic nuclei was carried out. Using optical, fluorescence, and atomic force microscopy, individual molecular and subcellular images of biological samples were detected. The mechanical properties of DNA molecules with and without bound-proteins were studied by applying small forces induced by surface tension or viscous drag and stretched DNA molecules. DNA molecules, modeled as random coil in solution, were straightened and immobilized on flat surface, and a stress-strain relationship was analyzed. Preliminary result suggests that the DNA molecules with bound-proteins behave differently in solution from the DNA molecules alone. By monitoring a process of nucleus formation, a linkage of a shape and a size of nuclei to a state of DNA and a stress field in a nucleus suspension was observed.
|Number of pages
|Critical Reviews in Biomedical Engineering
|Published - 1998
ASJC Scopus subject areas
- Biomedical Engineering