Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

M. B. Prystowsky; J. Ely; S. N. Vogel; E. Goldwasser; F. W. Fitch

Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

M. B. Prystowsky, J. Ely, S. N. Vogel, E. Goldwasser, F. W. Fitch

Research output: Contribution to journal › Article › peer-review

Abstract

The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 10⁸ U/mg of protein by using ion exchange high-pressure liquid chromatography.

Original language	English (US)
Pages (from-to)	2757-2761
Number of pages	5
Journal	Federation Proceedings
Volume	42
Issue number	10
State	Published - 1983
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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title = "Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte",

abstract = "The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 108 U/mg of protein by using ion exchange high-pressure liquid chromatography.",

author = "Prystowsky, {M. B.} and J. Ely and Vogel, {S. N.} and E. Goldwasser and Fitch, {F. W.}",

year = "1983",

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T1 - Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

AU - Prystowsky, M. B.

AU - Ely, J.

AU - Vogel, S. N.

AU - Goldwasser, E.

AU - Fitch, F. W.

PY - 1983

Y1 - 1983

N2 - The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 108 U/mg of protein by using ion exchange high-pressure liquid chromatography.

AB - The Mls-reactive murine helper T cell clone L2 produces at least 10 lymphokine activities affecting at least five distinct target cells. Culture conditions can be optimized to enable production of high levels of colony-stimulating factors (CSFs) by lectin stimulation of L2 cells under serum-free conditions. Selective enrichment of three lymphokine activities (interleukin 2, CSF, and interferon) can be achieved by using a combination of phenyl-Sepharose and hydroxyapatite chromatography. At least two types of CSF can be separated by concanavalin A-Sepharose chromatography. The CSF that does not bind to concanavalin A-Sepharose can be enriched to a specific activity of at least 5 x 108 U/mg of protein by using ion exchange high-pressure liquid chromatography.

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Biochemical enrichment of lymphokines secreted by a cloned helper T lymphocyte

Abstract

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