Biochemical detection of capsid in the nucleus during HIV-1 infection

Anastasia Selyutina, Felipe Diaz-Griffero

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid content as well as for nuclear and cytosolic markers to assess the bona fide origin of the fractions. This protocol can be applied in both cycling and non-cycling human cells. For complete details on the use and execution of this protocol, please refer to Selyutina et al. (2020a).

Original languageEnglish (US)
Article number100323
JournalSTAR Protocols
Issue number1
StatePublished - Mar 19 2021
Externally publishedYes


  • Cell biology
  • Molecular biology
  • Protein biochemistry

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology
  • General Medicine


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