Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells

Daniel R. Rines; Mariana Gomez; Yingyao Zhou; Paul DeJesus; Seanna Grob; Serge Batalov; Marc Labow; Dieter Huesken; Craig Mickanin; Jonathan Half; Mischa Reinhardt; Francois Natt; Joerg Lange; David J. Sharp; Sumit K. Chanda; Jeremy S. Caldwell

doi:10.1109/ISBI.2007.357051

Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells

Daniel R. Rines, Mariana Gomez, Yingyao Zhou, Paul DeJesus, Seanna Grob, Serge Batalov, Marc Labow, Dieter Huesken, Craig Mickanin, Jonathan Half, Mischa Reinhardt, Francois Natt, Joerg Lange, David J. Sharp, Sumit K. Chanda, Jeremy S. Caldwell

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We designed an image-based screen using automated microscopy to discover genetic factors essential for mitotic progression. Using a human genome-wide RNAi library, we performed a loss-offunction screen looking for siRNAs that arrest cells in metaphase. Supervised clustering of the multiparametric morphological data with gene ontology (GO) annotations, protein families, tissue expression and protein-protein interactions functionally classified these genes into discrete mitotic processes involved in spindle assembly. We used this approach to rapidly identify important novel genes based on their association with known genes in the clusters. We present here the automated methods used to identify these components.

Original language	English (US)
Title of host publication	2007 4th IEEE International Symposium on Biomedical Imaging
Subtitle of host publication	From Nano to Macro - Proceedings
Pages	1112-1115
Number of pages	4
DOIs	https://doi.org/10.1109/ISBI.2007.357051
State	Published - 2007
Event	2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07 - Arlington, VA, United States Duration: Apr 12 2007 → Apr 15 2007

Publication series

Name	2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings

Other

Other	2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07
Country/Territory	United States
City	Arlington, VA
Period	4/12/07 → 4/15/07

Keywords

Biological cells
Biomedical image processing
Biomedical imaging

ASJC Scopus subject areas

Electrical and Electronic Engineering
General Medicine

Access to Document

10.1109/ISBI.2007.357051

Cite this

Rines, D. R., Gomez, M., Zhou, Y., DeJesus, P., Grob, S., Batalov, S., Labow, M., Huesken, D., Mickanin, C., Half, J., Reinhardt, M., Natt, F., Lange, J., Sharp, D. J., Chanda, S. K., & Caldwell, J. S. (2007). Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells. In 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings (pp. 1112-1115). Article 4193485 (2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings). https://doi.org/10.1109/ISBI.2007.357051

Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells. / Rines, Daniel R.; Gomez, Mariana; Zhou, Yingyao et al.
2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings. 2007. p. 1112-1115 4193485 (2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Rines, DR, Gomez, M, Zhou, Y, DeJesus, P, Grob, S, Batalov, S, Labow, M, Huesken, D, Mickanin, C, Half, J, Reinhardt, M, Natt, F, Lange, J, Sharp, DJ, Chanda, SK & Caldwell, JS 2007, Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells. in 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings., 4193485, 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings, pp. 1112-1115, 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07, Arlington, VA, United States, 4/12/07. https://doi.org/10.1109/ISBI.2007.357051

Rines DR, Gomez M, Zhou Y, DeJesus P, Grob S, Batalov S et al. Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells. In 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings. 2007. p. 1112-1115. 4193485. (2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings). doi: 10.1109/ISBI.2007.357051

Rines, Daniel R. ; Gomez, Mariana ; Zhou, Yingyao et al. / Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells. 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings. 2007. pp. 1112-1115 (2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings).

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title = "Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells",

abstract = "We designed an image-based screen using automated microscopy to discover genetic factors essential for mitotic progression. Using a human genome-wide RNAi library, we performed a loss-offunction screen looking for siRNAs that arrest cells in metaphase. Supervised clustering of the multiparametric morphological data with gene ontology (GO) annotations, protein families, tissue expression and protein-protein interactions functionally classified these genes into discrete mitotic processes involved in spindle assembly. We used this approach to rapidly identify important novel genes based on their association with known genes in the clusters. We present here the automated methods used to identify these components.",

keywords = "Biological cells, Biomedical image processing, Biomedical imaging",

author = "Rines, {Daniel R.} and Mariana Gomez and Yingyao Zhou and Paul DeJesus and Seanna Grob and Serge Batalov and Marc Labow and Dieter Huesken and Craig Mickanin and Jonathan Half and Mischa Reinhardt and Francois Natt and Joerg Lange and Sharp, {David J.} and Chanda, {Sumit K.} and Caldwell, {Jeremy S.}",

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doi = "10.1109/ISBI.2007.357051",

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AU - Batalov, Serge

AU - Labow, Marc

AU - Huesken, Dieter

AU - Mickanin, Craig

AU - Half, Jonathan

AU - Reinhardt, Mischa

AU - Natt, Francois

AU - Lange, Joerg

AU - Sharp, David J.

AU - Chanda, Sumit K.

AU - Caldwell, Jeremy S.

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Automated microscopy screen to identify components required for mitotic cell cycle progression in human cells

Abstract

Publication series

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Keywords

ASJC Scopus subject areas

Access to Document

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