Assessment of antibacterial and free radical scavenging activity in psychrophilic Arthrobacter sp

Rajiv Pathak, Ruby Singh, Avnika Singh, Hemlata Gautam, Atlar Singh Dhaker, Raj Kumar, Rajesh Arora, Hemant K. Gautam

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Present investigation was undertaken to examine a psychrophilic bacterial species isolated from the high altitude region of Leh (India) as a potential sources of antioxidant and antibacterial properties. Extraction of secondary metabolites was done by using Diaion HP-20 adsorbent resin and elution was done with methanol. The methanol extracts were evaluated for their anti oxidant activity by DPPH radical scavenging assay and antibacterial activity was measured by using Kirby bauer method (disk diffusion method). In this report, methanolic extracts of Arthrobacter sp. was tested against Dietzia sp K44, Micrococcus flavus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella typhi and Micrococcus luteus for their antibacterial properties. Methanol extracts were further analysed by UV-Vis spectroscopy and high performance liquid chromatography (HPLC). To the best of our knowledge, this is the first report demonstrating the antibacterial and antioxidant properties in psychrophilic Arthrobacter sp.

Original languageEnglish (US)
Pages (from-to)344-355
Number of pages12
StatePublished - 2011
Externally publishedYes


  • Antimicrobial assay
  • DPPH antioxidant assay
  • Extraction of metabolites

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery


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