@article{d8b33afc1e524f24b9378060e7dad257,
title = "An Xist-activating antisense RNA required for X-chromosome inactivation",
abstract = "The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.",
author = "Sarkar, {Mrinal K.} and Srimonta Gayen and Surinder Kumar and Emily Maclary and Emily Buttigieg and Michael Hinten and Archana Kumari and Clair Harris and Takashi Sado and Sundeep Kalantry",
note = "Funding Information: We thank David Burke and the members of the Kalantry lab for discussions and critical review of the manuscript. We acknowledge the services of the University of Michigan Sequencing Core Facility, supported in part by the University of Michigan Comprehensive Cancer Center. This work was funded by an NIH National Research Service Award #5-T32-GM07544 from the National Institute of General Medicine Sciences to E.M.; a University of Michigan Reproductive Sciences Program training grant, an NIH National Research Service Award 1F31HD080280-01 from the National Institute of Child Health and Human Development (E.M.); a Rackham Predoctoral Fellowship from the University of Michigan (E.M.); an NIH T32 Career Training in Reproductive Biology Training Grant from the NICHD (M.H.); an NIH Director{\textquoteright}s New Innovator Award (DP2-OD-008646-01) to S.K.; and by a Basil O{\textquoteright}Connor Starter Scholar Research Award Grant (5-FY12-119) from the March of Dimes Foundation. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited.",
year = "2015",
month = oct,
day = "19",
doi = "10.1038/ncomms9564",
language = "English (US)",
volume = "6",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
}