An Xist-activating antisense RNA required for X-chromosome inactivation

Mrinal K. Sarkar, Srimonta Gayen, Surinder Kumar, Emily Maclary, Emily Buttigieg, Michael Hinten, Archana Kumari, Clair Harris, Takashi Sado, Sundeep Kalantry

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

Original languageEnglish (US)
Article number8564
JournalNature communications
StatePublished - Oct 19 2015
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)


Dive into the research topics of 'An Xist-activating antisense RNA required for X-chromosome inactivation'. Together they form a unique fingerprint.

Cite this