TY - JOUR
T1 - An integrative review of mechanotransduction in endothelial, epithelial (renal) and dendritic cells (osteocytes)
AU - Weinbaum, Sheldon
AU - Duan, Yi
AU - Thi, Mia M.
AU - You, Lidan
N1 - Funding Information:
This work was supported by National Institute of Health grants HL44485 (endothelium), DK-62289 (renal), and AR48699 and AR057139 (bone).
PY - 2011/12
Y1 - 2011/12
N2 - In this review we will examine from a biomechanical and ultrastructural viewpoint how the cytoskeletal specialization of three basic cell types, endothelial cells (ECs), epithelial cells (renal tubule) and dendritic cells (osteocytes), enables the mechano-sensing of fluid flow in both their native in vivo environment and in culture, and the downstream signaling that is initiated at the molecular level in response to fluid flow. These cellular responses will be discussed in terms of basic mysteries and paradoxes encountered by each cell type. In ECs fluid shear stress (FSS) is nearly entirely attenuated by the endothelial glycocalyx that covers their apical membrane and yet FSS is communicated to both intracellular and junctional molecular components in activating a wide variety of signaling pathways. The same is true in proximal tubule (PT) cells where a dense brush border of microvilli covers the apical surface and the flow at the apical membrane is negligible. A four decade old unexplained mystery is the ability of PT epithelia to reliably reabsorb 60% of the flow entering the tubule regardless of the glomerular filtration rate. In the cortical collecting duct (CCD) the flow rates are so low that a special sensing apparatus, a primary cilia is needed to detect very small variations in tubular flow. In bone it has been a century old mystery as to how osteocytes embedded in a stiff mineralized tissue are able to sense miniscule whole tissue strains that are far smaller than the cellular level strains required to activate osteocytes in vitro.
AB - In this review we will examine from a biomechanical and ultrastructural viewpoint how the cytoskeletal specialization of three basic cell types, endothelial cells (ECs), epithelial cells (renal tubule) and dendritic cells (osteocytes), enables the mechano-sensing of fluid flow in both their native in vivo environment and in culture, and the downstream signaling that is initiated at the molecular level in response to fluid flow. These cellular responses will be discussed in terms of basic mysteries and paradoxes encountered by each cell type. In ECs fluid shear stress (FSS) is nearly entirely attenuated by the endothelial glycocalyx that covers their apical membrane and yet FSS is communicated to both intracellular and junctional molecular components in activating a wide variety of signaling pathways. The same is true in proximal tubule (PT) cells where a dense brush border of microvilli covers the apical surface and the flow at the apical membrane is negligible. A four decade old unexplained mystery is the ability of PT epithelia to reliably reabsorb 60% of the flow entering the tubule regardless of the glomerular filtration rate. In the cortical collecting duct (CCD) the flow rates are so low that a special sensing apparatus, a primary cilia is needed to detect very small variations in tubular flow. In bone it has been a century old mystery as to how osteocytes embedded in a stiff mineralized tissue are able to sense miniscule whole tissue strains that are far smaller than the cellular level strains required to activate osteocytes in vitro.
KW - Actin cortical web
KW - Actin filament bundles
KW - Bone cell processes
KW - Brush border microvilli
KW - Cortical collecting duct
KW - Endothelial glycocalyx
KW - Integrin attachments
KW - Lacunar-canalicular system
KW - Proximal tubule
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U2 - 10.1007/s12195-011-0179-6
DO - 10.1007/s12195-011-0179-6
M3 - Review article
AN - SCOPUS:84856457221
SN - 1865-5025
VL - 4
SP - 510
EP - 537
JO - Cellular and Molecular Bioengineering
JF - Cellular and Molecular Bioengineering
IS - 4
ER -