An improved imaging system that corrects MS2-induced RNA destabilization

Weihan Li; Anna Maekiniemi; Hanae Sato; Christof Osman; Robert H. Singer

doi:10.1038/s41592-022-01658-1

An improved imaging system that corrects MS2-induced RNA destabilization

Weihan Li, Anna Maekiniemi, Hanae Sato, Christof Osman, Robert H. Singer

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.

Original language	English (US)
Pages (from-to)	1558-1562
Number of pages	5
Journal	Nature Methods
Volume	19
Issue number	12
DOIs	https://doi.org/10.1038/s41592-022-01658-1
State	Published - Dec 2022

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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abstract = "The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.",

author = "Weihan Li and Anna Maekiniemi and Hanae Sato and Christof Osman and Singer, {Robert H.}",

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AU - Maekiniemi, Anna

AU - Sato, Hanae

AU - Osman, Christof

AU - Singer, Robert H.

PY - 2022/12

Y1 - 2022/12

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AB - The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.

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An improved imaging system that corrects MS2-induced RNA destabilization

Abstract

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