An improved imaging system that corrects MS2-induced RNA destabilization

Weihan Li, Anna Maekiniemi, Hanae Sato, Christof Osman, Robert H. Singer

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.

Original languageEnglish (US)
Pages (from-to)1558-1562
Number of pages5
JournalNature Methods
Issue number12
StatePublished - Dec 2022

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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