@article{5aa12db257bd424cb95d5d10393fb6a7,
title = "An improved imaging system that corrects MS2-induced RNA destabilization",
abstract = "The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.",
author = "Weihan Li and Anna Maekiniemi and Hanae Sato and Christof Osman and Singer, {Robert H.}",
note = "Funding Information: The authors thank A. Jacobson, U.T. Meier, R.A. Coleman and the members of the Singer laboratory for insightful discussions. The authors also thank X. Meng for her help with cloning. This work was supported by American Heart Association Postdoctoral Fellowship 903024 (W.L.), 1R35 GM136296-01 (R.H.S.) and the European Research Council ERCStG-714739 IlluMitoDNA (C.O.). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = dec,
doi = "10.1038/s41592-022-01658-1",
language = "English (US)",
volume = "19",
pages = "1558--1562",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "12",
}