Amplification of the biotin-avidin immunofluorescence technique

Joan W. Berman, Ross S. Basch

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


An amplification of the immunofluorescence technique which uses biotinylated antibody and fluoresceinated avidin is described. By introducing a sandwich technique using fluorescein-conjuated goat ant-avidin, a 5-fold enhancement of staining over the conventional immunofluorescence method is achieved, and the brightness is more than twice that achieved with the simple biotin-fluoresceinated avidin reaction.

Original languageEnglish (US)
Pages (from-to)335-338
Number of pages4
JournalJournal of Immunological Methods
Issue number3-4
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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