TY - JOUR
T1 - Amebic colitis in an antigenically and serologically negative patient
T2 - usefulness of a small-subunit ribosomal RNA gene-based polymerase chain reaction in diagnosis
AU - Solaymani-Mohammadi, Shahram
AU - Coyle, Christina M.
AU - Factor, Stephen M.
AU - Petri, William A.
N1 - Funding Information:
This study was supported by the National Institutes of Health grant AI-43596 to W.A.P. S.S.-M was a visiting researcher at the Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, VA, and his stay in UVa (University of Virginia Health System, Charlottesville, VA) was partly funded by a scholarship from the Iranian Ministry of Health and Medical Education.
PY - 2008/11
Y1 - 2008/11
N2 - Specific identification of Entamoeba histolytica in clinical specimens is an essential confirmatory diagnostic step in the management of amebiasis. Here, we report an unusual case of amebic colitis in a 20-year-old female immigrant from South China. The patient had experienced diarrhea, crampy abdominal pain, and fever for approximately 3 weeks prior to admission to hospital and had treated herself at home with metronidazole. On admission, stool microscopy and serology for E. histolytica were negative. Because the clinical findings raised the suspicion of Clostridium difficile fulminant colitis, she underwent a subtotal colectomy. Histopathology revealed flask-shaped ulcers characteristic of amebic colitis. Consequently, E. histolytica DNA was detected by a sensitive small-subunit rRNA polymerase chain reaction (PCR) from feces, and the patient was successfully treated for amebiasis with metronidazole. This case exemplifies the relative insensitivity of serologic tests for the diagnosis of intestinal amebiasis and the difficulties encountered in detecting the parasite antigen in a patient partially treated with metronidazole. We conclude that when the possibility of invasive intestinal amebiasis is suspected, detecting the parasite DNA directly in the stool sample by PCR using E. histolytica-specific primers may be an alternative, noninvasive, and reliable tool for the specific diagnosis of the disease.
AB - Specific identification of Entamoeba histolytica in clinical specimens is an essential confirmatory diagnostic step in the management of amebiasis. Here, we report an unusual case of amebic colitis in a 20-year-old female immigrant from South China. The patient had experienced diarrhea, crampy abdominal pain, and fever for approximately 3 weeks prior to admission to hospital and had treated herself at home with metronidazole. On admission, stool microscopy and serology for E. histolytica were negative. Because the clinical findings raised the suspicion of Clostridium difficile fulminant colitis, she underwent a subtotal colectomy. Histopathology revealed flask-shaped ulcers characteristic of amebic colitis. Consequently, E. histolytica DNA was detected by a sensitive small-subunit rRNA polymerase chain reaction (PCR) from feces, and the patient was successfully treated for amebiasis with metronidazole. This case exemplifies the relative insensitivity of serologic tests for the diagnosis of intestinal amebiasis and the difficulties encountered in detecting the parasite antigen in a patient partially treated with metronidazole. We conclude that when the possibility of invasive intestinal amebiasis is suspected, detecting the parasite DNA directly in the stool sample by PCR using E. histolytica-specific primers may be an alternative, noninvasive, and reliable tool for the specific diagnosis of the disease.
KW - Amebic colitis
KW - Clostridium difficile
KW - Entamoeba histolytica
KW - SSU-rRNA
KW - Serologic test
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U2 - 10.1016/j.diagmicrobio.2008.06.014
DO - 10.1016/j.diagmicrobio.2008.06.014
M3 - Article
C2 - 18691843
AN - SCOPUS:54049112982
SN - 0732-8893
VL - 62
SP - 333
EP - 335
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -