Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption

Guoxiang Xie, Wei Zhong, Houkai Li, Qiong Li, Yunping Qiu, Xiaojiao Zheng, Huiyuan Chen, Xueqing Zhao, Shucha Zhang, Zhanxiang Zhou, Steven H. Zeisel, Wei Jia

Research output: Contribution to journalArticlepeer-review

147 Scopus citations


Our understanding of the bile acid metabolism is limited by the fact that previous analyses have primarily focused on a selected few circulating bile acids; the bile acid profiles of the liver and gastrointestinal tract pools are rarely investigated. Here, we determined how chronic ethanol consumption altered the bile acids in multiple body compartments (liver, gastrointestinal tract, and serum) of rats. Rats were fed a modified Lieber-DeCarli liquid diet with 38% of calories as ethanol (the amount equivalent of 4-5 drinks in humans). While conjugated bile acids predominated in the liver (98.3%), duodenum (97.8%), and ileum (89.7%), unconjugated bile acids comprised the largest proportion of measured bile acids in serum (81.2%), the cecum (97.7%), and the rectum (97.5%). In particular, taurine-conjugated bile acids were significantly decreased in the liver and gastrointestinal tract of ethanol-treated rats, while unconjugated and glycineconjugated species increased. Ethanol consumption caused increased expression of genes involved in bile acid biosynthesis, efflux transport, and reduced expression of genes regulating bile acid influx transport in the liver. These results provide an improved understanding of the systemic modulations of bile acid metabolism in mammals through the gut-liver axis.-Xie, G., Zhong, W., Li, H., Li, Q., Qiu, Y., Zheng, X., Chen, H., Zhao, X., Zhang, S., Zhou, Z., Zeisel, S. H., Jia, W. Alteration of bile acid metabolism in the rat induced by chronic ethanol consumption.

Original languageEnglish (US)
Pages (from-to)3583-3593
Number of pages11
JournalFASEB Journal
Issue number9
StatePublished - Sep 2013
Externally publishedYes


  • Blood
  • Gastrointestinal tract
  • Liver
  • Metabolomics
  • Ultraperformance liquid chromatography mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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