Abstract
It was investigated whether in vivo aging of rat liver is associated with changes in the induction and rate of disappearance of DNA damage. For this purpose 6- and 36-month-old rats were intraperitoneally injected with a single, low dose (5 mg/kg body wt.) of the model liver carcinogen 2-acetylaminofluorene (AAF). Using the 32P-postlabeling assay we found that N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) was the major DNA-adduct formed. The minor adduct N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) could only be detected after doses of 20 mg/kg or more. Quantitation of adduct levels at various time points after treatment indicated a rapid induction of AF-adducts, which were already present at 6 h after treatment. The subsequent loss of AF-adducts was relatively slow, as was indicated by the presence of a substantial amount of AF-adducts as late as 21 days after treatment. Slight age-related differences in the pattern of induction and disappearance of AF-adducts and a somewhat higher level of persisting lesions in old than in young rats were observed.
Original language | English (US) |
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Pages (from-to) | 373-384 |
Number of pages | 12 |
Journal | Chemico-Biological Interactions |
Volume | 69 |
Issue number | 4 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
Keywords
- 2-Acetylaminofluorene
- Aging
- DNA repair
- Persisting adducts
- Rat liver
ASJC Scopus subject areas
- Toxicology