Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells

E. Mullaart; M. E.T.I. Boerrigter; A. Brouwer; F. Berends; J. Vijg

doi:10.1016/0047-6374(88)90017-6

Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells

E. Mullaart, M. E.T.I. Boerrigter, A. Brouwer, F. Berends, J. Vijg

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.

Original language	English (US)
Pages (from-to)	41-49
Number of pages	9
Journal	Mechanisms of Ageing and Development
Volume	45
Issue number	1
DOIs	https://doi.org/10.1016/0047-6374(88)90017-6
State	Published - Oct 1988
Externally published	Yes

Keywords

Accumulation of DNA damage
Aging
Alkaline elution
Rat liver cells

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(88)90017-6

Cite this

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title = "Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells",

abstract = "The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.",

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AU - Mullaart, E.

AU - Boerrigter, M. E.T.I.

AU - Brouwer, A.

AU - Berends, F.

AU - Vijg, J.

PY - 1988/10

Y1 - 1988/10

N2 - The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.

AB - The amount of spontaneous damage in the DNA of rat liver cells was measured by using the alkaline elution assay. An age-related increase of approximately 700 detectable alkali-labile sites (80%) was found for rat parenchymal liver cells; cells from 6-month-old rats contained approximately 900 alkali-labile sites per cell while cells from 36-month-old rats contained approximately 1600 alkali-labile sites. In contrast to the situation with the postmitotic parenchymal liver cells, no age-related increase in the number of alkali-labile sites was found for the non-parenchymal liver cell fraction, which has a higher mitotic activity. These results support the hypothesis that aging takes place predominantly in postmitotic cells.

KW - Accumulation of DNA damage

KW - Aging

KW - Alkaline elution

KW - Rat liver cells

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Age-dependent accumulation of alkali-labile sites in DNA of post-mitotic but not in that of mitotic rat liver cells

Abstract

Keywords

ASJC Scopus subject areas

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