TY - JOUR
T1 - Advances in lectin microarray technology
T2 - optimized protocols for piezoelectric print conditions
AU - Pilobello, Kanoelani T.
AU - Agrawal, Praveen
AU - Rouse, Richard
AU - Mahal, Lara K.
N1 - Publisher Copyright:
© 2013 by John Wiley & Sons, Inc.
PY - 2013
Y1 - 2013
N2 - Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N-linked, O-linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate-binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N-linked, α- and β-galactose and GalNAc, α-2,3- and α-2,6-sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non-contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed.
AB - Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N-linked, O-linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate-binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N-linked, α- and β-galactose and GalNAc, α-2,3- and α-2,6-sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non-contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed.
KW - Nano‐Plotter
KW - carbohydrate analysis
KW - glycomics
KW - lectin microarray
KW - piezoelectric
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U2 - 10.1002/9780470559277.ch120035
DO - 10.1002/9780470559277.ch120035
M3 - Article
C2 - 23788322
AN - SCOPUS:84896504473
SN - 2160-4762
VL - 5
SP - 1
EP - 23
JO - Current protocols in chemical biology
JF - Current protocols in chemical biology
IS - 1
ER -