Actin dynamics in lamellipodia of migrating border cells in the Drosophila ovary revealed by a GFP-actin fusion protein

Vladislav V. Verkhusha, Shoichiro Tsukita, Hiroki Oda

Research output: Contribution to journalArticlepeer-review

100 Scopus citations


Directional migration of border cells in the Drosophila egg chambers is a developmentally regulated event that requires dynamic cellular functions. In this study, the electron microscopic observation of migrating border cells revealed loose actin bundles in forepart lamellipodia and numerous microvilli extending from nurse cells and providing multiple adhesive contacts with border cells. To analyze the dynamics of actin in migrating border cells in vivo, we constructed a green fluorescent protein-actin fusion protein and induced its expression in Drosophila using the GAL4/UAS system. The green fluorescent protein-actin was incorporated into the actin bundles and it enabled visualization of the rapid cytoskeletal changes in border cell lamellipodia. During the growth of the lamellipodia, the actin bundles that increased in number and size radiated from the bundle-organizing center. Quantification of the fluorescence intensity showed that an accumulation of bundle-associated and spotted green fluorescent protein-actin signals took place during their centripetal movement. Our results favored a treadmilling model for actin behavior in border cell lamellipodia. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalFEBS Letters
Issue number2-3
StatePublished - Feb 26 1999
Externally publishedYes


  • Actin dynamics
  • BC, border cell
  • BDM, 2,3-butanedione monoxime
  • BOC, bundle-organizing center
  • Border cell migration
  • Forepart lamellipodium
  • GFP, green fluorescent protein
  • Green fluorescent protein-actin fusion
  • NC, nurse cell

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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