Absence of reproducibly detectable low-level HIV viremia in highly exposed seronegative men and women

Eric Delwart, Flavien Bernardin, Tzong Hae Lee, Valerie Winkelman, Chenglong Liu, Haynes Sheppard, Albert Liu, Ruth Greenblatt, Katryn Anastos, Jack Dehovitz, Marek Nowicki, Mardge Cohen, Elizabeth T. Golub, Jason Barbour, Susan Buchbinder, Michael P. Busch

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Objective: Transient HIV infections have been invoked to account for the cellular immune responses detected in highly virus-exposed individuals who have remained HIV-seronegative. We tested for very low levels of HIV RNA in 524 seronegative plasma samples from 311 highly exposed women and men from three longitudinal HIV cohorts. Design: Two thousand and seventy-three transcription-mediated amplification (TMA) HIV RNA tests were performed for an average of 3.95 TMA assays per plasma sample. Quadruplicate TMA assays, analyzing a total of 2 ml of plasma, provided an estimated sensitivity of 3.5 HIV RNA copies/ml. Results: Four samples from individuals who did not seroconvert within the following 6 months were positive for HIV RNA. For one sample, human polymorphism DNA analysis indicated a sample mix-up. Borderline HIV RNA detection signals were detected for the other three positive samples but further replicate TMA testing yielded no positive results. Nested PCR assays (n = 254) for HIV proviral DNA in peripheral blood mononuclear cells (PBMCs) from these three individuals were negative. Conclusion: Transient viremia was not reproducibly detected in highly HIV-exposed seronegative men and women. If transient infections do occur, plasma HIV RNA levels may remain below the detection limits of the sensitive assay used here, be of very short duration, or viral replication may be restricted to mucosal surfaces or their draining lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)619-623
Number of pages5
Issue number5
StatePublished - Mar 13 2011


  • acute infections
  • preseroconversion
  • transcription-mediated amplification
  • transient infections
  • window phase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'Absence of reproducibly detectable low-level HIV viremia in highly exposed seronegative men and women'. Together they form a unique fingerprint.

Cite this